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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 Jun;83(11):3958–3962. doi: 10.1073/pnas.83.11.3958

Cotransfer of the Ed alpha and Ad beta genes into L cells results in the surface expression of a functional mixed-isotype Ia molecule.

B Malissen, N Shastri, M Pierres, L Hood
PMCID: PMC323644  PMID: 3086873

Abstract

Ia molecules play a key role in antigen recognition by T lymphocytes. To analyze the structural features of the individual alpha and beta chains relevant to the assembly of intact Ia molecules, mouse fibroblasts were cotransfected with various combinations of haplotype- and isotype-mismatched Ia alpha/beta gene pairs. Two important points emerged. First, the level of surface expression of a given haplotype-mismatched A alpha A beta pair appears to depend upon the alpha and beta chain alleles involved. Second, transfection with some isotype-mismatched combinations such as Ed alpha Ad beta results in a significant level of surface expression of a stable mixed-isotype dimer, which also appears to be normally expressed at a low level by an Iad-positive B lymphoma. Moreover, a T-cell hybridoma specific for human gamma globulin and restricted by the Ed molecule was found to be efficiently stimulated by the Ed alpha Ad beta-positive transfectant in the presence of antigen. The stimulation was specifically inhibited by monoclonal antibodies directed to either the Ia or the L3T4 molecule. These findings suggest that the estimates of the potential number of Ia molecules available in an animal for restricting T-lymphocyte recognition of antigens must be revised.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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