Abstract
The physiological importance of translational regulation in controlling the synthesis of ribosomal proteins from the L11 ribosomal protein operon was determined for the classical regulatory phenomena of growth rate dependence and stringent control. Translational regulation of the L11 operon by ribosomal protein L1, the L11 operon-specific translational repressor protein, was abolished by introducing a chromosomal mutation that causes an alteration of the site where L1 interacts with L11 operon mRNA. It was found that abolishing translational regulation of the L11 operon also abolished growth-rate-dependent regulation and stringent control of the L11 operon ribosomal proteins without affecting the normal regulation of ribosomal proteins from other operons that are not regulated by L1. These results show that both growth-rate-dependent control and stringent control of ribosomal protein synthesis in the L11 operon are a direct result of translational regulation.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baughman G., Nomura M. Localization of the target site for translational regulation of the L11 operon and direct evidence for translational coupling in Escherichia coli. Cell. 1983 Oct;34(3):979–988. doi: 10.1016/0092-8674(83)90555-x. [DOI] [PubMed] [Google Scholar]
- Baughman G., Nomura M. Translational regulation of the L11 ribosomal protein operon of Escherichia coli: analysis of the mRNA target site using oligonucleotide-directed mutagenesis. Proc Natl Acad Sci U S A. 1984 Sep;81(17):5389–5393. doi: 10.1073/pnas.81.17.5389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dabbs E. R. The ribosomal components responsible for kasugamycin dependence, and its suppression, in a mutant of Escherichia coli. Mol Gen Genet. 1980 Jan;177(2):271–276. doi: 10.1007/BF00267438. [DOI] [PubMed] [Google Scholar]
- Dennis P. P., Nomura M. Stringent control of ribosomal protein gene expression in Escherichia coli. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3819–3823. doi: 10.1073/pnas.71.10.3819. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fallon A. M., Jinks C. S., Strycharz G. D., Nomura M. Regulation of ribosomal protein synthesis in Escherichia coli by selective mRNA inactivation. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3411–3415. doi: 10.1073/pnas.76.7.3411. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freedman L. P., Zengel J. M., Lindahl L. Genetic dissection of stringent control and nutritional shift-up response of the Escherichia coli S10 ribosomal protein operon. J Mol Biol. 1985 Oct 20;185(4):701–712. doi: 10.1016/0022-2836(85)90055-5. [DOI] [PubMed] [Google Scholar]
- Gausing K. Regulation of ribosome production in Escherichia coli: synthesis and stability of ribosomal RNA and of ribosomal protein messenger RNA at different growth rates. J Mol Biol. 1977 Sep 25;115(3):335–354. doi: 10.1016/0022-2836(77)90158-9. [DOI] [PubMed] [Google Scholar]
- Gourse R. L., de Boer H. A., Nomura M. DNA determinants of rRNA synthesis in E. coli: growth rate dependent regulation, feedback inhibition, upstream activation, antitermination. Cell. 1986 Jan 17;44(1):197–205. doi: 10.1016/0092-8674(86)90498-8. [DOI] [PubMed] [Google Scholar]
- Jinks-Robertson S., Gourse R. L., Nomura M. Expression of rRNA and tRNA genes in Escherichia coli: evidence for feedback regulation by products of rRNA operons. Cell. 1983 Jul;33(3):865–876. doi: 10.1016/0092-8674(83)90029-6. [DOI] [PubMed] [Google Scholar]
- Maher D. L., Dennis P. P. In vivo transcription of E. coli genes coding for rRNA, ribosomal proteins and subunits of RNA polymerase: influence of the stringent control system. Mol Gen Genet. 1977 Oct 20;155(2):203–211. doi: 10.1007/BF00393161. [DOI] [PubMed] [Google Scholar]
- Miura A., Krueger J. H., Itoh S., de Boer H. A., Nomura M. Growth-rate-dependent regulation of ribosome synthesis in E. coli: expression of the lacZ and galK genes fused to ribosomal promoters. Cell. 1981 Sep;25(3):773–782. doi: 10.1016/0092-8674(81)90185-9. [DOI] [PubMed] [Google Scholar]
- Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nomura M., Gourse R., Baughman G. Regulation of the synthesis of ribosomes and ribosomal components. Annu Rev Biochem. 1984;53:75–117. doi: 10.1146/annurev.bi.53.070184.000451. [DOI] [PubMed] [Google Scholar]
- Parsons G. D., Mackie G. A. Expression of the gene for ribosomal protein S20: effects of gene dosage. J Bacteriol. 1983 Apr;154(1):152–160. doi: 10.1128/jb.154.1.152-160.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shapira S. K., Chou J., Richaud F. V., Casadaban M. J. New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase. Gene. 1983 Nov;25(1):71–82. doi: 10.1016/0378-1119(83)90169-5. [DOI] [PubMed] [Google Scholar]
- Siehnel R. J., Morgan E. A. Unbalanced rRNA gene dosage and its effects on rRNA and ribosomal-protein synthesis. J Bacteriol. 1985 Aug;163(2):476–486. doi: 10.1128/jb.163.2.476-486.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Singer P., Nomura M. Stability of ribosomal protein mRNA and translational feedback regulation in Escherichia coli. Mol Gen Genet. 1985;199(3):543–546. doi: 10.1007/BF00330773. [DOI] [PubMed] [Google Scholar]
- UMBARGER H. E., BROWN B. Isoleucine and valine metabolism in Escherichia coli. V. Antagonism between isoleucine and valine. J Bacteriol. 1955 Aug;70(2):241–248. doi: 10.1128/jb.70.2.241-248.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zengel J. M., Archer R. H., Freedman L. P., Lindahl L. Role of attenuation in growth rate-dependent regulation of the S10 r-protein operon of E. coli. EMBO J. 1984 Jul;3(7):1561–1565. doi: 10.1002/j.1460-2075.1984.tb02011.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zengel J. M., Lindahl L. Transcriptional control of the S10 ribosomal protein operon of Escherichia coli after a shift to higher temperature. J Bacteriol. 1985 Jul;163(1):140–147. doi: 10.1128/jb.163.1.140-147.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zoller M. J., Smith M. Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors. Methods Enzymol. 1983;100:468–500. doi: 10.1016/0076-6879(83)00074-9. [DOI] [PubMed] [Google Scholar]