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. 1986 Jun;83(12):4451–4455. doi: 10.1073/pnas.83.12.4451

Keratinocyte-derived T-cell growth factor: a T-cell growth factor functionally distinct from interleukin 2.

T S Kupper, D L Coleman, J McGuire, D Goldminz, M C Horowitz
PMCID: PMC323751  PMID: 3520573

Abstract

T-cell growth factor, more recently termed interleukin 2 (IL-2), is the product of activated T lymphocytes and is considered the principal trophic factor for T lymphocytes. The activity of IL-2 preparations is assessed by the degree to which they support the growth of various IL-2-dependent cell lines. We report that murine epidermal epithelial cells (keratinocytes) produce and release a factor that supports the growth of the helper-T-cell-derived, IL-2-dependent cell line HT-2. This substance, keratinocyte-derived T-cell growth factor (KTGF), does not support the growth of an IL-2-dependent cell line derived from cytotoxic T cells (line CTLL-2). This differential effect on IL-2-dependent cell lines is unique to KTGF. KTGF has an apparent molecular weight of 25,000-35,000 and has properties similar to those of conventional IL-2 by reversed-phase and gel-filtration HPLC analysis. However, even highly purified KTGF fails to stimulate the proliferation of CTLL-2 cells. The observation that epidermal epithelium produces a trophic factor for T lymphocytes may help explain the basis for preferential proliferation of T cells in the microenvironment of skin in certain dermatologic disorders. Further, it suggests that different IL-2-dependent T-cell lines may have distinct growth requirements and that non-lymphocyte cell types may produce factors capable of maintaining the growth of T cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ansel J. C., Luger T. A., Green I. The effect of in vitro and in vivo UV irradiation on the production of ETAF activity by human and murine keratinocytes. J Invest Dermatol. 1983 Dec;81(6):519–523. doi: 10.1111/1523-1747.ep12522862. [DOI] [PubMed] [Google Scholar]
  2. Baker P. E., Gillis S., Smith K. A. Monoclonal cytolytic T-cell lines. J Exp Med. 1979 Jan 1;149(1):273–278. doi: 10.1084/jem.149.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cantrell D. A., Smith K. A. The interleukin-2 T-cell system: a new cell growth model. Science. 1984 Jun 22;224(4655):1312–1316. doi: 10.1126/science.6427923. [DOI] [PubMed] [Google Scholar]
  4. Chu A. C., Patterson J. A., Goldstein G., Berger C. L., Takezaki S., Edelson R. L. Thymopoietin-like substance in human skin. J Invest Dermatol. 1983 Sep;81(3):194–197. doi: 10.1111/1523-1747.ep12517686. [DOI] [PubMed] [Google Scholar]
  5. Edelson R. L. Cutaneous T-cell lymphomas: clues of a skin-thymus interaction. J Invest Dermatol. 1976 Sep;67(3):419–424. doi: 10.1111/1523-1747.ep12514718. [DOI] [PubMed] [Google Scholar]
  6. Granstein R. D., Lowy A., Greene M. I. Epidermal antigen-presenting cells in activation of suppression: identification of a new functional type of ultraviolet radiation-resistant epidermal cell. J Immunol. 1984 Feb;132(2):563–565. [PubMed] [Google Scholar]
  7. Haynes B. F., Hensley L. L., Jegasothy B. V. Differentiation of human T lymphocytes: II. Phenotypic difference in skin and blood malignant T-cells in cutaneous T-cell lymphoma. J Invest Dermatol. 1982 Apr;78(4):323–326. doi: 10.1111/1523-1747.ep12507406. [DOI] [PubMed] [Google Scholar]
  8. Haynes B. F., Scearce R. M., Lobach D. F., Hensley L. L. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J Exp Med. 1984 Apr 1;159(4):1149–1168. doi: 10.1084/jem.159.4.1149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Honda M., Chan C., Shevach E. M. Characterization and partial purification of a specific interleukin 2 inhibitor. J Immunol. 1985 Sep;135(3):1834–1839. [PubMed] [Google Scholar]
  10. Kashima N., Nishi-Takaoka C., Fujita T., Taki S., Yamada G., Hamuro J., Taniguchi T. Unique structure of murine interleukin-2 as deduced from cloned cDNAs. 1985 Jan 31-Feb 6Nature. 313(6001):402–404. doi: 10.1038/313402a0. [DOI] [PubMed] [Google Scholar]
  11. Katz S. I., Tamaki K., Sachs D. H. Epidermal Langerhans cells are derived from cells originating in bone marrow. Nature. 1979 Nov 15;282(5736):324–326. doi: 10.1038/282324a0. [DOI] [PubMed] [Google Scholar]
  12. Kaye J., Janeway C. A., Jr Induction of receptors for interleukin 2 requires T cell Ag:Ia receptor crosslinking and interleukin 1. Lymphokine Res. 1984 Summer;3(4):175–182. [PubMed] [Google Scholar]
  13. Kaye J., Porcelli S., Tite J., Jones B., Janeway C. A., Jr Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells. J Exp Med. 1983 Sep 1;158(3):836–856. doi: 10.1084/jem.158.3.836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Luger T. A., Stadler B. M., Katz S. I., Oppenheim J. J. Epidermal cell (keratinocyte)-derived thymocyte-activating factor (ETAF). J Immunol. 1981 Oct;127(4):1493–1498. [PubMed] [Google Scholar]
  15. Luger T. A., Stadler B. M., Luger B. M., Mathieson B. J., Mage M., Schmidt J. A., Oppenheim J. J. Murine epidermal cell-derived thymocyte-activating factor resembles murine interleukin 1. J Immunol. 1982 May;128(5):2147–2152. [PubMed] [Google Scholar]
  16. Luger T. A., Wirth U., Köck A. Epidermal cells synthesize a cytokine with interleukin 3-like properties. J Immunol. 1985 Feb;134(2):915–919. [PubMed] [Google Scholar]
  17. Maino V. C., Pace D. R. Induction of interleukin 2 from a murine B cell tumor by a factor found in immune serum. J Immunol. 1985 Apr;134(4):2419–2425. [PubMed] [Google Scholar]
  18. Patterson J. A., Edelson R. L. Cutaneous T cell lymphoma. Med Clin North Am. 1982 Jul;66(4):895–913. doi: 10.1016/s0025-7125(16)31400-6. [DOI] [PubMed] [Google Scholar]
  19. Rubenfeld M. R., Silverstone A. E., Knowles D. M., Halper J. P., De Sostoa A., Fenoglio C. M., Edelson R. L. Induction of lymphocyte differentiation by epidermal cultures. J Invest Dermatol. 1981 Aug;77(2):221–224. doi: 10.1111/1523-1747.ep12480029. [DOI] [PubMed] [Google Scholar]
  20. Sauder D. N., Carter C. S., Katz S. I., Oppenheim J. J. Epidermal cell production of thymocyte activating factor (ETAF). J Invest Dermatol. 1982 Jul;79(1):34–39. doi: 10.1111/1523-1747.ep12510569. [DOI] [PubMed] [Google Scholar]
  21. Sauder D. N., Monick M. M., Hunninghake G. W. Epidermal cell-derived thymocyte activating factor (ETAF) is a potent T-cell chemoattractant. J Invest Dermatol. 1985 Nov;85(5):431–433. doi: 10.1111/1523-1747.ep12277126. [DOI] [PubMed] [Google Scholar]
  22. Smith C. A., Rennick D. M. Characterization of a murine lymphokine distinct from interleukin 2 and interleukin 3 (IL-3) possessing a T-cell growth factor activity and a mast-cell growth factor activity that synergizes with IL-3. Proc Natl Acad Sci U S A. 1986 Mar;83(6):1857–1861. doi: 10.1073/pnas.83.6.1857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Smith K. A. Interleukin 2. Annu Rev Immunol. 1984;2:319–333. doi: 10.1146/annurev.iy.02.040184.001535. [DOI] [PubMed] [Google Scholar]
  24. Streilein J. W. Lymphocyte traffic, T-cell malignancies and the skin. J Invest Dermatol. 1978 Sep;71(3):167–171. doi: 10.1111/1523-1747.ep12547071. [DOI] [PubMed] [Google Scholar]
  25. Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J Exp Med. 1979 Dec 1;150(6):1510–1519. doi: 10.1084/jem.150.6.1510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yuspa S. H., Hawley-Nelson P., Koehler B., Stanley J. R. A survey of transformation markers in differentiating epidermal cell lines in culture. Cancer Res. 1980 Dec;40(12):4694–4703. [PubMed] [Google Scholar]

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