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. 1986 Jun;83(12):4552–4555. doi: 10.1073/pnas.83.12.4552

Retroviral transfer of genes into canine hemopoietic progenitor cells in culture: a model for human gene therapy.

W W Kwok, F Schuening, R B Stead, A D Miller
PMCID: PMC323772  PMID: 3459189

Abstract

Amphotropic retroviral vectors containing either a mutant dihydrofolate reductase gene (DHFR) or the bacterial neomycin phosphotransferase gene (neo) were used to infect canine hemopoietic cells. We report successful transfer and expression of the DHFR and neo genes in canine hemopoietic progenitor cells (colony-forming units, granulocyte/macrophage) as measured by the ability of the viruses to confer resistance to either methotrexate or the aminoglycoside G418, respectively. Transfer was achieved in the absence of helper virus by using retrovirus packaging cell lines. Successful transfer of these genes into canine hemopoietic progenitor cells in vitro indicates the feasibility of gene transfer into canine marrow for autologous reconstitution. Studies of transfer of new genetic information into a large, outbred animal such as the dog will provide a preclinical model for future gene therapy in humans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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