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. 1986 Jul;83(13):4636–4640. doi: 10.1073/pnas.83.13.4636

Identification of processing events in the synthesis of platelet-derived growth factor-like proteins by human osteosarcoma cells.

D T Graves, A J Owen, S R Williams, H N Antoniades
PMCID: PMC323796  PMID: 3460062

Abstract

The human osteosarcoma-derived cell line U-2 OS expresses c-sis mRNA and synthesizes platelet-derived growth factor (PDGF)-like proteins. Pulse-chase experiments indicate that proteins of 23 kDa and 180 kDa are synthesized first. The 23-kDa protein undergoes dimerization and proteolysis, giving rise to the 30-kDa dimeric protein secreted by the cells. The 180-kDa protein is proteolytically cleaved in a complex series of steps that give rise to several intracellular species. It is also the likely precursor of high molecular mass PDGF-like or PDGF-associated proteins secreted by these cells. The processing and secretion of the 180-kDa protein is slower than that of the 23-kDa protein. Subcellular fractionation and studies with the antibiotic monensin indicate that the processing events occur in the Golgi-endoplasmic reticulum compartment of U-2 OS cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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