Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 Jul;83(14):5291–5295. doi: 10.1073/pnas.83.14.5291

Production of a monoclonal antibody specific for seminomas and dysgerminomas.

D Bailey, R Baumal, J Law, K Sheldon, P Kannampuzha, M Stratis, H Kahn, A Marks
PMCID: PMC323937  PMID: 3523489

Abstract

A monoclonal antibody (M2A, IgG2a) was produced against a cultured human ovarian epithelial adenocarcinoma cell line, HEY. Monoclonal antibody M2A reacted with a glycoprotein of molecular weight 40,000 on the surface of HEY cells. The affinity constant of the monoclonal antibody M2A for HEY cells was 10(9) M-1, and the number of binding sites on HEY cells was 2 X 10(4) per cell. The monoclonal antibody produced positive immunoperoxidase staining of fetal (but not adult) testis and of seminomas and dysgerminomas but did not stain various normal adult tissues or other gonadal or extragonadal tumors. Monoclonal antibody M2A may be useful for confirming a histological diagnosis of seminoma and dysgerminoma.

Full text

PDF
5291

Images in this article

5293Image
on p.5293
5293Image
on p.5293
5293Image
on p.5293
5293Image
on p.5293
5293Image
on p.5293
5293Image
on p.5293
5294Image
on p.5294
5294Image
on p.5294
5294Image
on p.5294

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Battifora H., Sheibani K., Tubbs R. R., Kopinski M. I., Sun T. T. Antikeratin antibodies in tumor diagnosis. Distinction between seminoma and embryonal carcinoma. Cancer. 1984 Sep 1;54(5):843–848. doi: 10.1002/1097-0142(19840901)54:5<843::aid-cncr2820540514>3.0.co;2-g. [DOI] [PubMed] [Google Scholar]
  2. Bolivar I. Immunological crossreactivity of Paramecium surface antigens: conformational differences account for cell surface specificity. Proc Natl Acad Sci U S A. 1983 Feb;80(4):1003–1007. doi: 10.1073/pnas.80.4.1003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Buick R. N., Pullano R., Trent J. M. Comparative properties of five human ovarian adenocarcinoma cell lines. Cancer Res. 1985 Aug;45(8):3668–3676. [PubMed] [Google Scholar]
  4. Burk K. H., Oefinger P. E., Dreesman G. R. Detection of non-A, non-B hepatitis antigen by immunocytochemical staining. Proc Natl Acad Sci U S A. 1984 May;81(10):3195–3199. doi: 10.1073/pnas.81.10.3195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bush S. E., Martinez A., Bagshaw M. A. Primary mediastinal seminoma. Cancer. 1981 Oct 15;48(8):1877–1882. doi: 10.1002/1097-0142(19811015)48:8<1877::aid-cncr2820480827>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  6. Coffin C. M., Ewing S., Dehner L. P. Frequency of intratubular germ cell neoplasia with invasive testicular germ cell tumors. Histologic and immunocytochemical features. Arch Pathol Lab Med. 1985 Jun;109(6):555–559. [PubMed] [Google Scholar]
  7. Cohen C., Shulman G., Budgeon L. R. Immunohistochemical ferritin in testicular seminoma. Cancer. 1984 Nov 15;54(10):2190–2194. doi: 10.1002/1097-0142(19841115)54:10<2190::aid-cncr2820541020>3.0.co;2-2. [DOI] [PubMed] [Google Scholar]
  8. Frankel M. E., Gerhard W. The rapid determination of binding constants for antiviral antibodies by a radioimmunoassay. An analysis of the interaction between hybridoma proteins and influenza virus. Mol Immunol. 1979 Feb;16(2):101–106. doi: 10.1016/0161-5890(79)90051-8. [DOI] [PubMed] [Google Scholar]
  9. Houston R. R., Winter C. C., Smith J. P., Brenner M. A. Primary retroperitoneal seminoma without gonadal involvement: a report of 3 cases. J Urol. 1971 Dec;106(6):841–844. doi: 10.1016/s0022-5347(17)61414-9. [DOI] [PubMed] [Google Scholar]
  10. Kasper C. S., Schneider N. R., Childers J. H., Wilson J. D. Suprasellar germinoma. Unresolved problems in diagnosis, pathogenesis, and management. Am J Med. 1983 Oct;75(4):705–711. doi: 10.1016/0002-9343(83)90461-8. [DOI] [PubMed] [Google Scholar]
  11. Mahony J., Bose A., Cowdrey D., Nusair T., Lei M., Harris J., Marks A., Baumal R. A monoclonal antiidiotypic antibody to MOPC 315 IgA inhibits the growth of MOPC 315 myeloma cells in vitro. J Immunol. 1981 Jan;126(1):113–117. [PubMed] [Google Scholar]
  12. Marks A., Law J., Mahony J. B., Baumal R. The structural conservation of S100 protein during evolution: analysis by reactivity with a monoclonal antibody. J Neurochem. 1983 Jul;41(1):107–112. doi: 10.1111/j.1471-4159.1983.tb11820.x. [DOI] [PubMed] [Google Scholar]
  13. Miettinen M., Virtanen I., Talerman A. Intermediate filament proteins in human testis and testicular germ-cell tumors. Am J Pathol. 1985 Sep;120(3):402–410. [PMC free article] [PubMed] [Google Scholar]
  14. Mostofi F. K. Proceedings: Testicular tumors. Epidemiologic, etiologic, and pathologic features. Cancer. 1973 Nov;32(5):1186–1201. doi: 10.1002/1097-0142(197311)32:5<1186::aid-cncr2820320527>3.0.co;2-8. [DOI] [PubMed] [Google Scholar]
  15. Shulman M., Wilde C. D., Köhler G. A better cell line for making hybridomas secreting specific antibodies. Nature. 1978 Nov 16;276(5685):269–270. doi: 10.1038/276269a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES