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. 1980 Mar 25;8(6):1259–1272. doi: 10.1093/nar/8.6.1259

An improved direct RNA sequence method; its application to Vicia faba 5.8S ribosomal RNA.

Y Tanaka, T A Dyer, G G Brownlee
PMCID: PMC323990  PMID: 6159591

Abstract

We have developed a direct read-off sequencing procedure, based on the method of Stanley and Vassilenko using E. coli 5S ribosomal RNA as a model compound. Radioactive bands were transferred from an acrylamide gel fractionation in the first dimension onto a DEAE-cellulose thin layer plate. After in situ enzymatic digestion with RNase T2, mononucleoside 3',5'-diphosphates were separated in the second dimension by electrophoresis at pH 2.3. Using this two-dimensional procedure the entire sequence of 163 residues of the previously unknown Vicia faba (broad bean) 5.8S ribosomal RNA was deduced.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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