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. 1980 Apr 11;8(7):1675–1691. doi: 10.1093/nar/8.7.1675

Azidopolynucleotides as photoaffinity reagents.

I L Cartwright, D W Hutchinson
PMCID: PMC324024  PMID: 7001370

Abstract

Polynucleotides containing adenosine and 8-azidoadenosine or inosine and 8-azidoinosine residues have been prepared from mixtures of nucleoside diphosphates using polynucleotide phosphorylase from Escherichia coli. These copolymers can form complexes with polyuridylic or polycytidylic acids respectively. Single stranded poly(adenylic, 8-azidoadenylic acid) [poly(A,z8A)] has been used as a photoaffinity reagent to explore the subunit topography of RNA polymerase from E. coli.

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Selected References

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