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. 1980 Apr 25;8(8):1765–1782. doi: 10.1093/nar/8.8.1765

DNA sequence of the int-xis-Pi region of the bacteriophage lambda; overlap of the int and xis genes.

R W Davies
PMCID: PMC324034  PMID: 6253947

Abstract

The DNA sequence of the int and xis genes of lambda bacteriophage, as well as that of the PI promoter and a region of unknown function beyond this, was determined by the chain termination procedure. The Pribnow box sequence of the PI promoter lies just within the xis gene, and both possible sites of mRNA initiation from PI are within the xis gene. The end of the xis gene in its turn overlaps the start of the int gene by 23 base pairs, in a different reading frame. This overlap may play a role in ensuring efficient excision of the prophage in response to natural induction signals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Air G. M., Sanger F., Coulson A. R. Nucleotide and amino acid sequences of gene G of omegaX174. J Mol Biol. 1976 Dec 15;108(3):519–533. doi: 10.1016/s0022-2836(76)80134-9. [DOI] [PubMed] [Google Scholar]
  2. Barrell B. G., Air G. M., Hutchison C. A., 3rd Overlapping genes in bacteriophage phiX174. Nature. 1976 Nov 4;264(5581):34–41. doi: 10.1038/264034a0. [DOI] [PubMed] [Google Scholar]
  3. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davies R. W., Schreier P. H., Buchel D. E. Nucleotide sequence of the attachment site of coliphage lambda. Nature. 1977 Dec 22;270(5639):757–760. doi: 10.1038/270757a0. [DOI] [PubMed] [Google Scholar]
  5. Davies R. W., Schreier P. H., Büchel D. E. Determination of the endpoints of partial deletion mutants of the attachment site of bacteriophage lambda by DNA sequencing. Nucleic Acids Res. 1978 Sep;5(9):3209–3218. doi: 10.1093/nar/5.9.3209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davies R. W., Schreier P. H., Kotewicz M. L., Echols H. Studies on the binding of lambda Int protein to attachment site DNA: identification of a tight-binding site in the P' region. Nucleic Acids Res. 1979 Dec 20;7(8):2255–2273. doi: 10.1093/nar/7.8.2255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Echols H. Integrative and excisive recombination by bacteriophage lambda: evidence for an excision-specific recombination protein. J Mol Biol. 1970 Feb 14;47(3):575–583. doi: 10.1016/0022-2836(70)90324-4. [DOI] [PubMed] [Google Scholar]
  8. Enquist L. W., Weisberg R. A. A genetic analysis of the att-int-xis region of coliphage lambda. J Mol Biol. 1977 Apr;111(2):97–120. doi: 10.1016/s0022-2836(77)80117-4. [DOI] [PubMed] [Google Scholar]
  9. Gingery R., Echols H. Mutants of bacteriophage lambda unable to integrate into the host chromosome. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1507–1514. doi: 10.1073/pnas.58.4.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Guarneros G., Echols H. New mutants of bacteriophage lambda with a specific defect in excision from the host chromosome. J Mol Biol. 1970 Feb 14;47(3):565–574. doi: 10.1016/0022-2836(70)90323-2. [DOI] [PubMed] [Google Scholar]
  11. Haggerty D. M., Scheif R. F. Location in bacteriophage lamdba DNA of cleavage sites of the site-specific endonuclease from Bacillus amyloliquefaciens H. J Virol. 1976 May;18(2):659–663. doi: 10.1128/jvi.18.2.659-663.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kaiser A. D., Masuda T. Evidence for a prophage excision gene in lambda. J Mol Biol. 1970 Feb 14;47(3):557–564. doi: 10.1016/0022-2836(70)90322-0. [DOI] [PubMed] [Google Scholar]
  13. Katzir N., Oppenheim A., Belfort M., Oppenheim A. B. Activation of the lambda int gene by the cii and ciii gene products. Virology. 1976 Oct 15;74(2):324–331. doi: 10.1016/0042-6822(76)90339-1. [DOI] [PubMed] [Google Scholar]
  14. Landy A., Ross W. Viral integration and excision: structure of the lambda att sites. Science. 1977 Sep 16;197(4309):1147–1160. doi: 10.1126/science.331474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Murray K., Murray N. E. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. J Mol Biol. 1975 Nov 5;98(3):551–564. doi: 10.1016/s0022-2836(75)80086-6. [DOI] [PubMed] [Google Scholar]
  16. Nash H. A. Integration and excision of bacteriophage lambda. Curr Top Microbiol Immunol. 1977;78:171–199. doi: 10.1007/978-3-642-66800-5_6. [DOI] [PubMed] [Google Scholar]
  17. Pilacinski W., Mosharrafa E., Edmundson R., Zissler J., Fiandt M., Szybalski W. Insertion sequence IS2 associated with int-constitutive mutants of bacteriophage lambda. Gene. 1977;2(2):61–74. doi: 10.1016/0378-1119(77)90073-7. [DOI] [PubMed] [Google Scholar]
  18. Rosenberg M., Court D., Shimatake H., Brady C., Wulff D. L. The relationship between function and DNA sequence in an intercistronic regulatory region in phage lambda. Nature. 1978 Mar 30;272(5652):414–423. doi: 10.1038/272414a0. [DOI] [PubMed] [Google Scholar]
  19. Ross W., Landy A., Kikuchi Y., Nash H. Interaction of int protein with specific sites on lambda att DNA. Cell. 1979 Oct;18(2):297–307. doi: 10.1016/0092-8674(79)90049-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  21. Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schreier P. H., Davies R. W., Buchell D. E., Gronenborn B., Fanning T. G., von Wilcken B., Messing J. Precise location of the crossover region in the lambda attachment sequence. Nature. 1977 Jun 9;267(5611):555–557. doi: 10.1038/267555a0. [DOI] [PubMed] [Google Scholar]
  24. Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. II. Mutations induced by bacteriophage lambda in Escherichia coli K12. J Mol Biol. 1973 Oct 25;80(2):297–314. doi: 10.1016/0022-2836(73)90174-5. [DOI] [PubMed] [Google Scholar]
  25. Shine J., Dalgarno L. Terminal-sequence analysis of bacterial ribosomal RNA. Correlation between the 3'-terminal-polypyrimidine sequence of 16-S RNA and translational specificity of the ribosome. Eur J Biochem. 1975 Sep 1;57(1):221–230. doi: 10.1111/j.1432-1033.1975.tb02294.x. [DOI] [PubMed] [Google Scholar]
  26. Smith A. J. The use of exonuclease III for preparing single stranded DNA for use as a template in the chain terminator sequencing method. Nucleic Acids Res. 1979 Mar;6(3):831–848. doi: 10.1093/nar/6.3.831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Smith M., Brown N. L., Air G. M., Barrell B. G., Coulson A. R., Hutchison C. A., 3rd, Sanger F. DNA sequence at the C termini of the overlapping genes A and B in bacteriophage phi X174. Nature. 1977 Feb 24;265(5596):702–705. doi: 10.1038/265702a0. [DOI] [PubMed] [Google Scholar]
  28. Staden R. Further procedures for sequence analysis by computer. Nucleic Acids Res. 1978 Mar;5(3):1013–1016. doi: 10.1093/nar/5.3.1013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Staden R. Sequence data handling by computer. Nucleic Acids Res. 1977 Nov;4(11):4037–4051. doi: 10.1093/nar/4.11.4037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Steitz J. A., Jakes K. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734–4738. doi: 10.1073/pnas.72.12.4734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Thomas M., Davis R. W. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. doi: 10.1016/0022-2836(75)90383-6. [DOI] [PubMed] [Google Scholar]
  32. Zissler J. Integration-negative (int) mutants of phage lambda. Virology. 1967 Jan;31(1):189–189. doi: 10.1016/0042-6822(67)90030-x. [DOI] [PubMed] [Google Scholar]

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