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. 1980 Apr 25;8(8):1793–1804. doi: 10.1093/nar/8.8.1793

In vitro translation studies of the cytoplasmic nonpolysomal particles containing messenger RNA.

J P Liautard, J M Egly
PMCID: PMC324036  PMID: 7433129

Abstract

We analyzed the translational capacity of different kinds of free cytoplasmic messenger ribonucleoprotein complexes (free mRNP) in a Hela cell cell free system. Native free mRNP are not translated although free mRNP washed with 0.5 M KC1 can direct polypeptide synthesis. Furthermore, the 0.5 M KC1 wash possesses a factor which inhibits the translation of 0.5 M KC1 washed free mRNP as well as globin mRNA naked mRNA from plasmocytoma, or Hela cells. We also demonstrated that native free mRNP are able to form a complex with ribosomal subunits in the presence of initiation factors. This indicates that inhibition of translation by the 0.5 M KC1 wash occurs either at some point after initiation complex formation or at the elongation step.

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Selected References

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