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. 1980 May 10;8(9):1987–1997. doi: 10.1093/nar/8.9.1987

Selection and sequence analysis of a cDNA clone encoding a known chorion protein of the A family.

S G Tsitilou, J C Regier, F C Kafatos
PMCID: PMC324052  PMID: 7433133

Abstract

Using as criteria the size, abundance and developmental specificity of hybridizing mRNA sequences, we have selected from our chorion cDNA library a clone corresponding to a specific chorion protein, A4--cl. Comparison between the clone sequence and the largely known sequence of A4--cl validates the use of the cDNA library for sequence analysis of the chorion multigene families. The two major chorion protein families, A and B, share certain structural similarities.

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Selected References

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  1. Browne J. K., Paddock G. V., Liu A., Clarke P., Heindell H. C., Salser W. Nucleotide sequences from the rabbit beta globin gene inserted into Escherichia coli plasmids. Science. 1977 Jan 28;195(4276):389–391. doi: 10.1126/science.318762. [DOI] [PubMed] [Google Scholar]
  2. Drapeau G. R. Cleavage at glutamic acid with staphylococcal protease. Methods Enzymol. 1977;47:189–191. doi: 10.1016/0076-6879(77)47023-x. [DOI] [PubMed] [Google Scholar]
  3. Efstratiadis A., Kafatos F. C., Maniatis T. The primary structure of rabbit beta-globin mRNA as determined from cloned DNA. Cell. 1977 Apr;10(4):571–585. doi: 10.1016/0092-8674(77)90090-3. [DOI] [PubMed] [Google Scholar]
  4. Efstratiadis A., Vournakis J. N., Donis-Keller H., Chaconas G., Dougall D. K., Kafatos F. C. End labeling of enzymatically decapped mRNA. Nucleic Acids Res. 1977 Dec;4(12):4165–4174. doi: 10.1093/nar/4.12.4165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldsmith M. R., Basehoar G. Organization of the chorion genes of Bombyx mori, a multigene family. I. Evidence for linkage to chromosome 2. Genetics. 1978 Oct;90(2):291–310. doi: 10.1093/genetics/90.2.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hofstetter H., Schamböck A., Van Den Berg J., Weissmann C. Specific excision of the inserted DNA segment from hybrid plasmids constructed by the poly(dA). poly (dT) method. Biochim Biophys Acta. 1976 Dec 13;454(3):587–591. doi: 10.1016/0005-2787(76)90286-0. [DOI] [PubMed] [Google Scholar]
  8. Jones C. W., Rosenthal N., Rodakis G. C., Kafatos F. C. Evolution of two major chorion multigene families as inferred from cloned cDNA and protein sequences. Cell. 1979 Dec;18(4):1317–1332. doi: 10.1016/0092-8674(79)90242-3. [DOI] [PubMed] [Google Scholar]
  9. Kafatos F. C., Jones C. W., Efstratiadis A. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res. 1979 Nov 24;7(6):1541–1552. doi: 10.1093/nar/7.6.1541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kafatos F. C., Regier J. C., Mazur G. D., Nadel M. R., Blau H. M., Petri W. H., Wyman A. R., Gelinas R. E., Moore P. B., Paul M. The eggshell of insects: differentiation-specific proteins and the control of their synthesis and accumulation during development. Results Probl Cell Differ. 1977;8:45–145. doi: 10.1007/978-3-540-37332-2_2. [DOI] [PubMed] [Google Scholar]
  11. Lomedico P., Rosenthal N., Efstratidadis A., Gilbert W., Kolodner R., Tizard R. The structure and evolution of the two nonallelic rat preproinsulin genes. Cell. 1979 Oct;18(2):545–558. doi: 10.1016/0092-8674(79)90071-0. [DOI] [PubMed] [Google Scholar]
  12. Maniatis T., Kee S. G., Efstratiadis A., Kafatos F. C. Amplification and characterization of a beta-globin gene synthesized in vitro. Cell. 1976 Jun;8(2):163–182. doi: 10.1016/0092-8674(76)90001-5. [DOI] [PubMed] [Google Scholar]
  13. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nadel M. R., Kafatos F. C. Specific protein synthesis in cellular differentiation. IV. The chorion proteins of Bombyx mori and their program of synthesis. Dev Biol. 1980 Mar;75(1):26–40. doi: 10.1016/0012-1606(80)90141-4. [DOI] [PubMed] [Google Scholar]
  15. Paul M., Goldsmith M. R., Hunsley J. R., Kafatos F. C. Specific protein synthesis in cellular differentiation. Production of eggshell proteins by silkmoth follicular cells. J Cell Biol. 1972 Dec;55(3):653–680. doi: 10.1083/jcb.55.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Paul M., Kafaots F. C. Specific protein synthesis in cellular differentiation. II. The program of protein synthetic changes during chorion formation by slikmoth follicles, and its implementation in organ culture. Dev Biol. 1975 Jan;42(1):141–159. doi: 10.1016/0012-1606(75)90320-6. [DOI] [PubMed] [Google Scholar]
  17. Paul M., Kafatos F. C., Regier J. C. A comparative study of eggshell proteins in lepidoptera. J Supramol Struct. 1972;1(1):60–65. doi: 10.1002/jss.400010109. [DOI] [PubMed] [Google Scholar]
  18. Regier J. C., Kafatos F. C., Goodfliesh R., Hood L. Silkmoth chorion proteins: sequence analysis of the products of a multigene family. Proc Natl Acad Sci U S A. 1978 Jan;75(1):390–394. doi: 10.1073/pnas.75.1.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Regier J. C., Kafatos F. C., Kramer K. J., Heinrikson R. L., Keim P. S. Silkmoth chorion proteins. Their diversity, amino acid composition, and the NH-terminal sequence of one component. J Biol Chem. 1978 Feb 25;253(4):1305–1314. [PubMed] [Google Scholar]
  20. Sim G. K., Kafatos F. C., Jones C. W., Koehler M. D., Efstratiadis A., Maniatis T. Use of a cDNA library for studies on evolution and developmental expression of the chorion multigene families. Cell. 1979 Dec;18(4):1303–1316. doi: 10.1016/0092-8674(79)90241-1. [DOI] [PubMed] [Google Scholar]
  21. Vournakis J. N., Efstratiadis A., Kafatos F. C. Electrophoretic patterns of deadenylylated chorion and globin mRNAs. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2959–2963. doi: 10.1073/pnas.72.8.2959. [DOI] [PMC free article] [PubMed] [Google Scholar]

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