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. 1980 Jun 11;8(11):2489–2498. doi: 10.1093/nar/8.11.2489

Characterization of Mg2+-induced conformational change in the 50S ribosomal subunit by differential hydrogen exchange.

D Bonnet, E Begard, M Grunberg-Manago, G Hui Bon Hoa
PMCID: PMC324096  PMID: 7003535

Abstract

The technique of differential hydrogen exchange allows detection of a conformational change in the 50S subunit of Escherichia coli ribosome when the magnesium concentration is lowered in a range where ribosomal activity is fully preserved. This change is characterized by a seventy-fold acceleration of about thirty labile hydrogens in the case of a Mg2+ jump from 10 mM to 2 mM. The small number of hydrogens involved can explain the difficulty in detecting this change by other methods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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