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. 1980 Jun 11;8(11):2537–2546. doi: 10.1093/nar/8.11.2537

A bacterial cell that synthesizes a protein containing the antigenic determinants of rat prolactin.

C R Erwin, R A Maurer, J E Donelson
PMCID: PMC324100  PMID: 6160463

Abstract

Bacterial minicells containing three different recombinant plasmids with rat prolactin cDNA sequences inserted at the Pst I site of pBR322 via the poly(dG):poly(dC) joining technique were examined for the expression of rat prolactin antigenic determinants. The three prolactin coding sequences were in the same orientation as the coding sequence of the ampicillin-resistance gene of pBR322. The presence of each of the three recombinant plasmids induced some prolactin synthesis by the bacteria as measured by immunoprecipitation with anti-prolactin antisera. About 10% of the protein synthesized from one of the plasmids, prl 3, precipitated with the antisera. These prolactin antigenic determinants were part of a larger fused protein.

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Selected References

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