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. 1980 Jun 25;8(12):2725–2736. doi: 10.1093/nar/8.12.2725

Analysis of recombination in mammalian cells using SV40 genome segments having homologous overlapping termini.

P Upcroft, B Carter, C Kidson
PMCID: PMC324116  PMID: 6253890

Abstract

Segments of SV40 DNA having homologous overlapping termini recombine to produce viable genomes in monkey cells. Frequencies of recombination on either side of a deletion marker are non-random; replication and palindromes do not appear to be essential. Since recombination involves host enzymes, a suitable system has been devised for analysing host cell recombination functions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carbon J., Shenk T. E., Berg P. Biochemical procedure for production of small deletions in simian virus 40 DNA. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1392–1396. doi: 10.1073/pnas.72.4.1392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dubbs D. R., Rachmeler M., Kit S. Recombination between temperature-sensitive mutants of simian virus 40. Virology. 1974 Jan;57(1):161–174. doi: 10.1016/0042-6822(74)90117-2. [DOI] [PubMed] [Google Scholar]
  3. Farber F. E., Melnick J. L., Butel J. S. Optimal conditions for uptake of exogenous DNA by Chinese hamster lung cells deficient in hypoxanthine-guanine phosphoribosyltransferase. Biochim Biophys Acta. 1975 May 16;390(3):298–311. doi: 10.1016/0005-2787(75)90350-0. [DOI] [PubMed] [Google Scholar]
  4. Fiers W., Contreras R., Haegemann G., Rogiers R., Van de Voorde A., Van Heuverswyn H., Van Herreweghe J., Volckaert G., Ysebaert M. Complete nucleotide sequence of SV40 DNA. Nature. 1978 May 11;273(5658):113–120. doi: 10.1038/273113a0. [DOI] [PubMed] [Google Scholar]
  5. Ganem D., Nussbaum A. L., Davoli D., Fareed G. C. Isolation, propagation and characterization of replication requirements of reiteration mutants of Simian Virus 40. J Mol Biol. 1976 Feb 15;101(1):57–83. doi: 10.1016/0022-2836(76)90066-8. [DOI] [PubMed] [Google Scholar]
  6. Gruss P., Sauer G. Infectious linear DNA sequences replicating in simian virus 40-infected cells. J Virol. 1977 Feb;21(2):565–578. doi: 10.1128/jvi.21.2.565-578.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  8. Hotchkiss R. D. Models of genetic recombination. Annu Rev Microbiol. 1974;28(0):445–468. doi: 10.1146/annurev.mi.28.100174.002305. [DOI] [PubMed] [Google Scholar]
  9. Kelly T. J., Jr, Nathans D. The genome of simian virus 40. Adv Virus Res. 1977;21:85–173. doi: 10.1016/s0065-3527(08)60762-9. [DOI] [PubMed] [Google Scholar]
  10. Ketner G., Kelly T. J., Jr Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1102–1106. doi: 10.1073/pnas.73.4.1102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Landy A., Ruedisueli E., Robinson L., Foeller C., Ross W. Digestion of deoxyribonucleic acids from bacteriophage T7, lambda, and phi 80h with site-specific nucleases from Hemophilus influenzae strain Rc and strain Rd. Biochemistry. 1974 May 7;13(10):2134–2142. doi: 10.1021/bi00707a022. [DOI] [PubMed] [Google Scholar]
  12. Mertz J. E., Berg P. Defective simian virus 40 genomes: isolation and growth of individual clones. Virology. 1974 Nov;62(1):112–124. doi: 10.1016/0042-6822(74)90307-9. [DOI] [PubMed] [Google Scholar]
  13. Mertz J. E., Davis R. W. Cleavage of DNA by R 1 restriction endonuclease generates cohesive ends. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3370–3374. doi: 10.1073/pnas.69.11.3370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Peacock A. C., Dingman C. W. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. doi: 10.1021/bi00842a023. [DOI] [PubMed] [Google Scholar]
  15. Radding C. M. Genetic recombination: strand transfer and mismatch repair. Annu Rev Biochem. 1978;47:847–880. doi: 10.1146/annurev.bi.47.070178.004215. [DOI] [PubMed] [Google Scholar]
  16. Rosenberg H., Singer M., Rosenberg M. Highly reiterated sequences of SIMIANSIMIANSIMIANSIMIANSIMIAN. Science. 1978 Apr 28;200(4340):394–402. doi: 10.1126/science.205944. [DOI] [PubMed] [Google Scholar]
  17. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  18. Sleigh M. J., Topp W. C., Hanich R., Sambrook J. F. Mutants of SV40 with an altered small t protein are reduced in their ability to transform cells. Cell. 1978 May;14(1):79–88. doi: 10.1016/0092-8674(78)90303-3. [DOI] [PubMed] [Google Scholar]
  19. Smith D. I., Blattner F. R., Davies J. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii. Nucleic Acids Res. 1976 Feb;3(2):343–353. doi: 10.1093/nar/3.2.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sobell H. M. Molecular mechanism for genetic recombination. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2483–2487. doi: 10.1073/pnas.69.9.2483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  22. Subramanian K. N., Shenk T. Definition of the boundaries of the origin of DNA replication in simian virus 40. Nucleic Acids Res. 1978 Oct;5(10):3635–3642. doi: 10.1093/nar/5.10.3635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Thompson B. J., Sussman C. H., Warner R. C. Coinfection of E. coli with phages G4 and phiX174: origin of dimeric replicative form species. Virology. 1978 Jun 1;87(1):212–215. doi: 10.1016/0042-6822(78)90175-7. [DOI] [PubMed] [Google Scholar]
  24. Upcroft P., Skolnik H., Upcroft J. A., Solomon D., Khoury G., Hamer D. H., Fareed G. C. Transduction of a bacterial gene into mammalian cells. Proc Natl Acad Sci U S A. 1978 May;75(5):2117–2121. doi: 10.1073/pnas.75.5.2117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wagner R. E., Jr, Radman M. A mechanism for initiation of genetic recombination. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3619–3622. doi: 10.1073/pnas.72.9.3619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wilson J. H. Genetic analysis of host range mutant viruses suggests an uncoating defect in simian virus 40-resistant monkey cells. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3503–3507. doi: 10.1073/pnas.74.8.3503. [DOI] [PMC free article] [PubMed] [Google Scholar]

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