Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1980 Jul 11;8(13):2921–2937. doi: 10.1093/nar/8.13.2921

Transfer RNA genes of Drosophila melanogaster.

R Dudler, A H Egg, E Kubli, S Artavanis-Tsakonas, W J Gehring, R Steward, P Schedl
PMCID: PMC324135  PMID: 6776487

Abstract

Three recombinant plasmids containing randomly sheared genomic D. melanogaster tRNAs have been identified and characterized in detail. One of these, the plasmid 14C4, has a D. melanogaster (Dm) DNA segment of 18 kb, and has three tRNA2Arg and two tRNAAsN genes. The second plasmid, 38B10, has tRNAHis genes, while the third plasmid, 63H5, contains coding sequences for tRNA2Asp. The Dm DNA segments in each recombinant plasmid are derived from unique cytogenetic loci. 14C4 is from 84 F, 38B10 is from 48 F and 63H5 is from 70 A.

Full text

PDF
2921

Images in this article

2928Image
on p.2928
2933Image
on p.2933

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Artavanis-Tsakonas S., Schedl P., Tschudi C., Pirrotta V., Steward R., Gehring W. J. The 5S genes of Drosophila melanogaster. Cell. 1977 Dec;12(4):1057–1067. doi: 10.1016/0092-8674(77)90169-6. [DOI] [PubMed] [Google Scholar]
  2. Callan H. G. The organization of genetic units in chromosomes. J Cell Sci. 1967 Mar;2(1):1–7. doi: 10.1242/jcs.2.1.1. [DOI] [PubMed] [Google Scholar]
  3. Clarkson S. G., Birnstiel M. L., Serra V. Reiterated transfer RNA genes of Xenopus laevis. J Mol Biol. 1973 Sep 15;79(2):391–410. doi: 10.1016/0022-2836(73)90013-2. [DOI] [PubMed] [Google Scholar]
  4. Dahlberg A. E., Dingman C. W., Peacock A. C. Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels. J Mol Biol. 1969 Apr 14;41(1):139–147. doi: 10.1016/0022-2836(69)90131-4. [DOI] [PubMed] [Google Scholar]
  5. Dunn R., Delaney A. D., Gillam I. C., Hayashi S., Tener G. M., Grigliatti T., Misra V., Spurr M. G., Taylor D. M., Miller R. C., Jr Isolation and characterization of recombinant DNA plasmids carrying Drosophila tRNA genes. Gene. 1979 Nov;7(3-4):197–215. doi: 10.1016/0378-1119(79)90046-5. [DOI] [PubMed] [Google Scholar]
  6. Fedoroff N. V. On spacers. Cell. 1979 Apr;16(4):697–710. doi: 10.1016/0092-8674(79)90086-2. [DOI] [PubMed] [Google Scholar]
  7. Ford P. J., Brown R. D. Sequences of 5S ribosomal RNA from Xenopus mulleri and the evolution of 5S gene-coding sequences. Cell. 1976 Aug;8(4):485–493. doi: 10.1016/0092-8674(76)90216-6. [DOI] [PubMed] [Google Scholar]
  8. Fradin A., Gruhl H., Feldmann H. Mapping of yeast tRNAs by two-dimensional electrophoresis on polyacrylamide gels. FEBS Lett. 1975 Feb 1;50(2):185–189. doi: 10.1016/0014-5793(75)80485-6. [DOI] [PubMed] [Google Scholar]
  9. Grigliatti T. A., White B. N., Tener G. M., Kaufman T. C., Holden J. J., Suzuki D. T. Studies on the transfer RNA genes of Drosophila. Cold Spring Harb Symp Quant Biol. 1974;38:461–474. doi: 10.1101/sqb.1974.038.01.050. [DOI] [PubMed] [Google Scholar]
  10. Grigliatti T. A., White B. N., Tener G. M., Kaufman T. C., Suzuki D. T. The localization of transfer RNA5Lys genes in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3527–3531. doi: 10.1073/pnas.71.9.3527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hershey N. D., Conrad S. E., Sodja A., Yen P. H., Cohen M., Jr, Davidson N., Iigen C., Carbon J. The sequence arrangement of Drosophila melanogaster 5s DNA cloned in recombinant plasmids. Cell. 1977 Jul;11(3):585–598. doi: 10.1016/0092-8674(77)90076-9. [DOI] [PubMed] [Google Scholar]
  13. KIRBY K. S. A new method for the isolation of ribonucleic acids from mammalian tissues. Biochem J. 1956 Nov;64(3):405–408. doi: 10.1042/bj0640405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kedes L. H. Histone genes and histone messengers. Annu Rev Biochem. 1979;48:837–870. doi: 10.1146/annurev.bi.48.070179.004201. [DOI] [PubMed] [Google Scholar]
  15. Kelmers A. D., Heatherly D. E. Columns for rapid chromatographic separation of small amounts of tracer-labeled transfer ribonucleic acids. Anal Biochem. 1971 Dec;44(2):486–495. doi: 10.1016/0003-2697(71)90236-3. [DOI] [PubMed] [Google Scholar]
  16. Kelmers A. D., Novelli G. D., Stulberg M. P. Separation of transfer ribonucleic acids by reverse phase chromatography. J Biol Chem. 1965 Oct;240(10):3979–3983. [PubMed] [Google Scholar]
  17. Kern H. Fractionation of nucleic acids with special regard to rapidly labeled RNA. Anal Biochem. 1975 Jul;67(1):147–156. doi: 10.1016/0003-2697(75)90282-1. [DOI] [PubMed] [Google Scholar]
  18. Kubli E., Schmidt T. The localization of tRNA4Glu genes from Drosophila melanogaster by "in situ" hybridization. Nucleic Acids Res. 1978 May;5(5):1465–1478. doi: 10.1093/nar/5.5.1465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lobban P. E., Kaiser A. D. Enzymatic end-to end joining of DNA molecules. J Mol Biol. 1973 Aug 15;78(3):453–471. doi: 10.1016/0022-2836(73)90468-3. [DOI] [PubMed] [Google Scholar]
  20. McCutchan T. F., Gilham P. T., Söll D. An improved method for the purification of tRNA by chromatography on dihydroxyboryl substituted cellulose. Nucleic Acids Res. 1975 Jun;2(6):853–864. doi: 10.1093/nar/2.6.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Müller F., Clarkson S. G. Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA. Cell. 1980 Feb;19(2):345–353. doi: 10.1016/0092-8674(80)90509-7. [DOI] [PubMed] [Google Scholar]
  22. Prensky W. The radioiodination of RNA and DNA to high specific activities. Methods Cell Biol. 1976;13:121–152. doi: 10.1016/s0091-679x(08)61800-2. [DOI] [PubMed] [Google Scholar]
  23. Ritossa F. M., Atwood K. C., Lindsley D. L., Spiegelman S. On the chromosomal distribution of DNA complementary to ribosomal and soluble RNA. Natl Cancer Inst Monogr. 1966 Dec;23:449–472. [PubMed] [Google Scholar]
  24. Schmidt T., Egg A. H., Kubli E. The localization of tRNAAsp2 genes from Drosophila melanogaster by "in situ" hybridization. Mol Gen Genet. 1978 Sep 8;164(3):249–254. doi: 10.1007/BF00333153. [DOI] [PubMed] [Google Scholar]
  25. Smith G. P. Unequal crossover and the evolution of multigene families. Cold Spring Harb Symp Quant Biol. 1974;38:507–513. doi: 10.1101/sqb.1974.038.01.055. [DOI] [PubMed] [Google Scholar]
  26. Smith J. D. Transcription and processing of transfer RNA precursors. Prog Nucleic Acid Res Mol Biol. 1976;16:25–73. doi: 10.1016/s0079-6603(08)60755-2. [DOI] [PubMed] [Google Scholar]
  27. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  28. Steffensen D. M., Wimber D. E. Localization of tRNA genes in the salivary chromosomes of Drosophila by RNA:DNA hybridization. Genetics. 1971 Oct;69(2):163–178. doi: 10.1093/genetics/69.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Tartof K. D. Redundant genes. Annu Rev Genet. 1975;9:355–385. doi: 10.1146/annurev.ge.09.120175.002035. [DOI] [PubMed] [Google Scholar]
  30. Weber L., Berger E. Base sequence complexity of the stable RNA species of Drosophila melanogaster. Biochemistry. 1976 Dec 14;15(25):5511–5519. doi: 10.1021/bi00670a015. [DOI] [PubMed] [Google Scholar]
  31. Wellauer P. K., Dawid I. B. The structural organization of ribosomal DNA in Drosophila melanogaster. Cell. 1977 Feb;10(2):193–212. doi: 10.1016/0092-8674(77)90214-8. [DOI] [PubMed] [Google Scholar]
  32. White B. N., Tener G. M. Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locus. J Mol Biol. 1973 Mar 15;74(4):635–651. doi: 10.1016/0022-2836(73)90054-5. [DOI] [PubMed] [Google Scholar]
  33. White B. N., Tener G. M. Chromatography of Drosophila tRNA on BD-cellulose. Can J Biochem. 1973 Jun;51(6):896–902. doi: 10.1139/o73-111. [DOI] [PubMed] [Google Scholar]
  34. Yen P. H., Sodja A., Cohen M., Jr, Conrad S. E., Wu M., Davidson N. Sequence arrangement of tRNA genes on a fragment of Drosophila melanogaster DNA cloned in E. coli. Cell. 1977 Aug;11(4):763–777. doi: 10.1016/0092-8674(77)90290-2. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES