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. 1980 Jul 11;8(13):3043–3053. doi: 10.1093/nar/8.13.3043

The role of bacteriophage T7 gene 2 protein in DNA replication.

P Q Mooney, R North, I J Molineux
PMCID: PMC324143  PMID: 7001361

Abstract

The in vivo function of the gene 2 protein of bacteriophage T7 has been examined. The gene 2 protein appears to modulate the activity of the gene 3 endonuclease in order to prevent the premature degradation of any newly-formed DNA concatemers. This modulation is not however a direct interacton between the two proteins. In single-burst experiments rifamycin can substitute for the gene 2 protein, allowing formation of fast-sedimenting replicative DNA intermediates and progeny phage production. This suggests that the sole function of the gene 2 protein is inhibition of the host RNA polymerase and that the latter enzyme directs or promotes the endonucleolytic action of the gene 3 protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carlson K. Intracellular fate of deoxyribonucleic acid from T7 bacteriophages. J Virol. 1968 Oct;2(10):1230–1233. doi: 10.1128/jvi.2.10.1230-1233.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Center M. S. Replicative intermediates of bacteriophage T7 deoxyribonucleic acid. J Virol. 1972 Jul;10(1):115–123. doi: 10.1128/jvi.10.1.115-123.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Center M. S., Richardson C. C. An endonuclease induced after infection of Escherichia coli with bacteriophage T7. I. Purification and properties of the enzyme. J Biol Chem. 1970 Dec 10;245(23):6285–6291. [PubMed] [Google Scholar]
  4. Center M. S., Richardson C. C. An endonuclease induced after infection of Escherichia coli with bacteriophage T7. II. Specificity of the enzyme toward single- and double-stranded deoxyribonucleic acid. J Biol Chem. 1970 Dec 10;245(23):6292–6299. [PubMed] [Google Scholar]
  5. Center M. S. Role of gene 2 in bacteriophage T7 DNA synthesis. J Virol. 1975 Jul;16(1):94–100. doi: 10.1128/jvi.16.1.94-100.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chamberlin M. Isolation and characterization of prototrophic mutants of Escherichia coli unable to support the intracellular growth of T7. J Virol. 1974 Sep;14(3):509–516. doi: 10.1128/jvi.14.3.509-516.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DeWyngaert M. A., Hinkle D. C. Bacterial mutants affecting phage T7 DNA replication produce RNA polymerase resistant to inhibition by the T7 gene 2 protein. J Biol Chem. 1979 Nov 25;254(22):11247–11253. [PubMed] [Google Scholar]
  8. DeWyngaert M. A., Hinkle D. C. Characterization of the defects in bacteriophage T7 DNA synthesis during growth in the Escherichia coli mutant tsnB. J Virol. 1980 Feb;33(2):780–788. doi: 10.1128/jvi.33.2.780-788.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hesselbach B. A., Nakada D. "Host shutoff" function of bacteriophage T7: involvement of T7 gene 2 and gene 0.7 in the inactivation of Escherichia coli RNA polymerase. J Virol. 1977 Dec;24(3):736–745. doi: 10.1128/jvi.24.3.736-745.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ihler G. M., Thomas C. A., Jr Equal incorporation of both parental bacteriophage T7 deoxyribonucleic acid strands into intracellular concatemeric deoxyribonucleic acid. J Virol. 1970 Dec;6(6):877–880. doi: 10.1128/jvi.6.6.877-880.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kelly T. J., Jr, Thomas C. A., Jr An intermediate in the replication of bacteriophage T7 DNA molecules. J Mol Biol. 1969 Sep 28;44(3):459–475. doi: 10.1016/0022-2836(69)90373-8. [DOI] [PubMed] [Google Scholar]
  12. Kerr C., Sadowski P. D. The involvement of genes 3,4,5 and 6 in genetic recombination in bacteriophage T7. Virology. 1975 May;65(1):281–285. doi: 10.1016/0042-6822(75)90031-8. [DOI] [PubMed] [Google Scholar]
  13. LeClerc J. E., Richardson C. C. Gene 2 protein of bacteriophage T7: purification and requirement for packaging of T7 DNA in vitro. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4852–4856. doi: 10.1073/pnas.76.10.4852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Paetkau V., Langman L., Bradley R., Scraba D., Miller R. C., Jr Folded, concatenated genomes as replication intermediates of bacteriophage T7 DNA. J Virol. 1977 Apr;22(1):130–141. doi: 10.1128/jvi.22.1.130-141.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schlegel R. A., Thomas C. A., Jr Some special structural features of intracellular bacteriophage T7 concatemers. J Mol Biol. 1972 Jul 21;68(2):319–345. doi: 10.1016/0022-2836(72)90216-1. [DOI] [PubMed] [Google Scholar]
  16. Simon M. N., Studier F. W. Physical mapping of the early region of bacteriophage T7 DNA. J Mol Biol. 1973 Sep 15;79(2):249–265. doi: 10.1016/0022-2836(73)90004-1. [DOI] [PubMed] [Google Scholar]
  17. Strätling W., Krause E., Knippers R. Fast sedimenting deoxyribonucleic acid in bacteriophage T7-infected cells. Virology. 1973 Jan;51(1):109–119. doi: 10.1016/0042-6822(73)90371-1. [DOI] [PubMed] [Google Scholar]
  18. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  19. Studier F. W. Bacteriophage T7. Science. 1972 Apr 28;176(4033):367–376. doi: 10.1126/science.176.4033.367. [DOI] [PubMed] [Google Scholar]
  20. Studier F. W. Gene 0.3 of bacteriophage T7 acts to overcome the DNA restriction system of the host. J Mol Biol. 1975 May 15;94(2):283–295. doi: 10.1016/0022-2836(75)90083-2. [DOI] [PubMed] [Google Scholar]
  21. Studier F. W. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. doi: 10.1016/0042-6822(69)90104-4. [DOI] [PubMed] [Google Scholar]
  22. Watson J. D. Origin of concatemeric T7 DNA. Nat New Biol. 1972 Oct 18;239(94):197–201. doi: 10.1038/newbio239197a0. [DOI] [PubMed] [Google Scholar]

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