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. 1980 Aug 11;8(15):3459–3466. doi: 10.1093/nar/8.15.3459

Sequence-specific interaction of sigma subunit of E. coli RNA polymerase with DNA.

W Stender
PMCID: PMC324163  PMID: 7003537

Abstract

The interaction of sigma subunit of E. coli RNA polymerase with DNA, either double or single-stranded, and with two inhibitors of RNA synthesis was investigated by using antibodies directed against the subunit. Free sigma subunit was shown to interact with poly(dA), poly(dT), poly(dAC).poly(dGT), T7 DNA and, to a lesser degree, with lambda DNA. When the sigma subunit forms part of the holo enzyme, sigma also interacts with poly(dG).poly(dC). Rifampicin and streptolydigin interact with sigma in the holo enzyme and with free and core bound sigma subunit, respectively. The results suggest that sigma recognizes mainly AC-GT-sequences in double-stranded DNA. The findings are correlated with the base composition in RNA polymerase binding regions of promoters and suggest at least a general interaction between sigma subunit and single-stranded DNA in open complexes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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