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. 1980 Aug 25;8(16):3575–3589. doi: 10.1093/nar/8.16.3575

Relationship between the messenger RNAs transcribed from two overlapping genes of influenza virus.

S C Inglis, M J Gething, C M Brown
PMCID: PMC324176  PMID: 7433101

Abstract

The relationship of the mRNAs encoding the NS1 and NS2 polypeptides of influenza virus has been investigated through synthesis and characterisation of complementary DNA copies of the mRNAs. Previous work had shown that both mRNAs are encoded by virion RNA segment 8, and that the sequences comprising the smaller of the two mRNAs (the NS2 mRNA) were also present on the NS1 mRNA. Our results indicate that the mRNA encoding the NS2 polypeptide of the avian influenza, fowl plague virus, is approximately 400 ntds long, and that its sequences correspond largely with the 3'-terminal region of the NS1 mRNA.

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Selected References

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  1. Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barry R. D., Mahy B. W. The influenza virus genome and its replication. Br Med Bull. 1979 Jan;35(1):39–46. doi: 10.1093/oxfordjournals.bmb.a071540. [DOI] [PubMed] [Google Scholar]
  3. Berk A. J., Sharp P. A. Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274–1278. doi: 10.1073/pnas.75.3.1274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  5. Brownlee G. G., Cartwright E. M. Rapid gel sequencing of RNA by primed synthesis with reverse transcriptase. J Mol Biol. 1977 Jul;114(1):93–117. doi: 10.1016/0022-2836(77)90285-6. [DOI] [PubMed] [Google Scholar]
  6. Caton A. J., Robertson J. S. New procedure for the production of influenza virus-specific double-stranded DNA's. Nucleic Acids Res. 1979 Nov 24;7(6):1445–1456. doi: 10.1093/nar/7.6.1445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chow L. T., Gelinas R. E., Broker T. R., Roberts R. J. An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA. Cell. 1977 Sep;12(1):1–8. doi: 10.1016/0092-8674(77)90180-5. [DOI] [PubMed] [Google Scholar]
  8. Efstratiadis A., Vournakis J. N., Donis-Keller H., Chaconas G., Dougall D. K., Kafatos F. C. End labeling of enzymatically decapped mRNA. Nucleic Acids Res. 1977 Dec;4(12):4165–4174. doi: 10.1093/nar/4.12.4165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Godson G. N., Roberts R. J. dna, single stranded/*metab. Virology. 1976 Sep;73(2):561–567. doi: 10.1016/0042-6822(76)90421-9. [DOI] [PubMed] [Google Scholar]
  10. Hay A. J., Abraham G., Skehel J. J., Smith J. C., Fellner P. Influenza virus messenger RNAs are incomplete transcripts of the genome RNAs. Nucleic Acids Res. 1977 Dec;4(12):4197–4209. doi: 10.1093/nar/4.12.4197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hay A. J., Lomniczi B., Bellamy A. R., Skehel J. J. Transcription of the influenza virus genome. Virology. 1977 Dec;83(2):337–355. doi: 10.1016/0042-6822(77)90179-9. [DOI] [PubMed] [Google Scholar]
  12. Inglis S. C., Barrett T., Brown C. M., Almond J. W. The smallest genome RNA segment of influenza virus contains two genes that may overlap. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3790–3794. doi: 10.1073/pnas.76.8.3790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Inglis S. C., Carroll A. R., Lamb R. A., Mahy B. W. Polypeptides specified by the influenza virus genome I. Evidence for eight distinct gene products specified by fowl plague virus. Virology. 1976 Oct 15;74(2):489–503. doi: 10.1016/0042-6822(76)90355-x. [DOI] [PubMed] [Google Scholar]
  14. Inglis S. C., Mahy B. W. Polypeptides specified by the influenza virus genome. 3. Control of synthesis in infected cells. Virology. 1979 May;95(1):154–164. doi: 10.1016/0042-6822(79)90410-0. [DOI] [PubMed] [Google Scholar]
  15. Inglis S. C., McGeoch D. J., Mahy B. W. Polypeptides specified by the influenza virus genoma. 2. Assignement of protein coding functions to individual genome segments by in vitro translation. Virology. 1977 May 15;78(2):522–536. doi: 10.1016/0042-6822(77)90128-3. [DOI] [PubMed] [Google Scholar]
  16. Krug R. M., Broni B. A., Bouloy M. Are the 5' ends of influenza viral mRNAs synthesized in vivo donated by host mRNAs? Cell. 1979 Oct;18(2):329–334. doi: 10.1016/0092-8674(79)90052-7. [DOI] [PubMed] [Google Scholar]
  17. Lamb R. A., Choppin P. W., Chanock R. M., Lai C. J. Mapping of the two overlapping genes for polypeptides NS1 and NS2 on RNA segment 8 of influenza virus genome. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1857–1861. doi: 10.1073/pnas.77.4.1857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lamb R. A., Choppin P. W. Segment 8 of the influenza virus genome is unique in coding for two polypeptides. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4908–4912. doi: 10.1073/pnas.76.10.4908. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lockard R. E., Alzner-Deweerd B., Heckman J. E., MacGee J., Tabor M. W., RajBhandary U. L. Sequence analysis of 5'[32P] labeled mRNA and tRNA using polyacrylamide gel electrophoresis. Nucleic Acids Res. 1978 Jan;5(1):37–56. doi: 10.1093/nar/5.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Palese P. The genes of influenza virus. Cell. 1977 Jan;10(1):1–10. doi: 10.1016/0092-8674(77)90133-7. [DOI] [PubMed] [Google Scholar]
  21. Ritchey M. B., Palese P., Schulman J. L. Mapping of the influenza virus genome. III. Identification of genes coding for nucleoprotein, membrane protein, and nonstructural protein. J Virol. 1976 Oct;20(1):307–313. doi: 10.1128/jvi.20.1.307-313.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Robertson J. S. 5' and 3' terminal nucleotide sequences of the RNA genome segments of influenza virus. Nucleic Acids Res. 1979 Aug 24;6(12):3745–3757. doi: 10.1093/nar/6.12.3745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rowlands D. J., Harris T. J., Brown F. More precise location of the polycytidylic acid tract in foot and mouth disease virus RNA. J Virol. 1978 May;26(2):335–343. doi: 10.1128/jvi.26.2.335-343.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]
  25. Skehel J. J. Polypeptide synthesis in influenza virus-infected cells. Virology. 1972 Jul;49(1):23–36. doi: 10.1016/s0042-6822(72)80004-7. [DOI] [PubMed] [Google Scholar]
  26. Sleigh M. J., Both G. W., Brownlee G. G. A new method for the size estimation of the RNA genome segments of influenza virus. Nucleic Acids Res. 1979 Apr;6(4):1309–1321. doi: 10.1093/nar/6.4.1309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sutcliffe J. G. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res. 1978 Aug;5(8):2721–2728. doi: 10.1093/nar/5.8.2721. [DOI] [PMC free article] [PubMed] [Google Scholar]

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