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. 1980 Aug 25;8(16):3721–3728. doi: 10.1093/nar/8.16.3721

Site-specific DNA-affinity chromatography of the lac repressor.

G Herrick
PMCID: PMC324186  PMID: 7001362

Abstract

To test the feasibility of site-specific DNA-affinity chromatography, E. coli lac repressor was bound to an operator-containing DNA column, and in parallel to a non-operator DNA column. Salt gradient elution shows: 1) elution from non-operator DNA was near 250mM KCl or NaCl; interpretation of this result suggests the usefulness of the procedure for studying salt-dependence of DNA-protein affinities; 2) elution from operator-containing DNA was delayed (average elution = 1000mM salt), demonstrating a feasibility of site-specific DNA-affinity chromatography, if one provides a sufficiently favorable ratio of specific to non-specific DNA binding sites; 3) repressor eluted from operator-containing DNA over a very broad salt range, which may represent chromatography-generated repressor heterogeneity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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