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. 1980 Sep 11;8(17):3895–3907. doi: 10.1093/nar/8.17.3895

The ribosome binding sites recognized by E. coli ribosomes have regions with signal character in both the leader and protein coding segments.

G F Scherer, M D Walkinshaw, S Arnott, D J Morré
PMCID: PMC324202  PMID: 7003539

Abstract

Oligonucleotide analysis, by a novel computerized procedure, was first applied to determine the sequence of an ideal E. coli promoter (Scherer et al., Nucl. Acids Res. 1978, 5:3759-3773) and has now been used to obtain the sequence of nucleotides that should be present in a messenger RNA for optimum binding to the E. coli ribosome. This sequence is: UU.UUAAAAAUUAAGGAGGUAUAUUAUGAAAAAAAUUAAAAAACUCAA AA U A AUA A CUC G. Comparison of this sequence with each of the 68 ribosome binding site sequences used to generate it shows a preference rather than an absolute requirement for a specific base in any given position. The preference for certain bases persists along the whole length of the RNA within the ribosome binding domain even though nearly half of that length includes translated codons. Thus messages without leader sequences (like lambda CI mRNA) can still have some affinity for the ribosome. Part of the model sequence is complementary to the 3'end of 16S rRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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