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. 1980 Sep 25;8(18):4057–4074. doi: 10.1093/nar/8.18.4057

Synthesis of human fibroblast interferon by E. coli.

D V Goeddel, H M Shepard, E Yelverton, D Leung, R Crea, A Sloma, S Pestka
PMCID: PMC324219  PMID: 6159584

Abstract

A cDNA library was constructed using mRNA from human fibroblasts induced with poly(I):poly(C). A bacterial clone containing fibroblast interferon cDNA sequences was identified by hybridization to a cDNA probe synthesized using deoxyoligonucleotide primers which hybridize to fibroblast interferon mRNA specifically. Expression plasmids were constructed which permitted the synthesis in E. coli of 8 x 10(7) units of human fibroblast interferon per liter of culture. The bacterially produced fibroblast interferon is indistinguishable from authentic human fibroblast interferon by several criteria.

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Selected References

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