Abstract
Of the three proteins, L5, L18 and L25, which bind to 5S RNA, the former two effect the interaction of 5S RNA with 23S RNA. We have used trypsin as a probe to investigate the roles of the proteins in this RNA-RNA assembly, with the following results: (1) In complexes with 5S RNA, the highly basic N-terminal region of L18 is accessible to trypsin. This accessibility is unaffected by L25. However, its presence is essential for stimulating L5 binding. (2) In 5S RNA-protein-23S RNA complexes proteins L5 and L18 are both strongly resistant to proteolysis. (3) No 5S RNA-23S RNA complex formation occurs in the presence of L5 and the C-terminal L18 fragment. Two possible models for the mechanism of RNA-RNA assembly are proposed.
Full text
PDF











Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aubert M., Bellemare G., Monier R. Selective reaction of glyoxal with guanine residues in native and denatured Escherichia coli 5S RNA. Biochimie. 1973;55(2):135–142. doi: 10.1016/s0300-9084(73)80385-2. [DOI] [PubMed] [Google Scholar]
- Bear D. G., Schleich T., Noller H. F., Garrett R. A. Alteration of 5S RNA conformation by ribosomal proteins L18 and L25. Nucleic Acids Res. 1977 Jul;4(7):2511–2526. doi: 10.1093/nar/4.7.2511. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chen-Schmeisser U., Garrett R. A. A new method for the isolation of a 5 S RNA complex with proteins L5, L18 and L25 from Escherichia coli ribosomes. FEBS Lett. 1977 Mar 1;74(2):287–291. doi: 10.1016/0014-5793(77)80866-1. [DOI] [PubMed] [Google Scholar]
- Chen R., Ehrke G. The primary structure of the 5 S RNA binding protein L5 of Escherichia coli ribosomes. FEBS Lett. 1976 Oct 15;69(1):240–245. doi: 10.1016/0014-5793(76)80695-3. [DOI] [PubMed] [Google Scholar]
- Douthwaite S., Garrett R. A., Wagner R., Feunteun J. A ribonuclease-resistant region of 5S RNA and its relation to the RNA binding sites of proteins L18 and L25. Nucleic Acids Res. 1979 Jun 11;6(7):2453–2470. doi: 10.1093/nar/6.7.2453. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox G. E., Woese C. R. 5S RNA secondary structure. Nature. 1975 Aug 7;256(5517):505–507. doi: 10.1038/256505a0. [DOI] [PubMed] [Google Scholar]
- Fox J. W., Wong K. P. Changes in the conformation and stability of 5 S RNA upon the binding of ribosomal proteins. J Biol Chem. 1978 Jan 10;253(1):18–20. [PubMed] [Google Scholar]
- Fuenteun J., Monier R., Garrett R., Le Bret M., Le Pecq J. B. Effect of 50 S subunit proteins L5, L18 and L25 on the fluorescence of 5 S RNA-bound ethidium bromide. J Mol Biol. 1975 Apr 25;93(4):535–541. doi: 10.1016/0022-2836(75)90245-4. [DOI] [PubMed] [Google Scholar]
- Garrett R. A., Noller H. F. Structures of complexes of 5S RNA with ribosomal proteins L5, L18 and L25 from Escherichia coli: identification of kethoxal-reactive sites on the 5S RNA. J Mol Biol. 1979 Aug 25;132(4):637–648. doi: 10.1016/0022-2836(79)90379-6. [DOI] [PubMed] [Google Scholar]
- Gray P. N., Garrett R. A., Stoffler G., Monier R. An attempt at the identification of the proteins involved in the incorporation of 5-S RNA during 50-S ribosomal subunit assembly. Eur J Biochem. 1972 Jul 24;28(3):412–421. doi: 10.1111/j.1432-1033.1972.tb01927.x. [DOI] [PubMed] [Google Scholar]
- Gray P. N., Monier R. Formation of a complex between 23 S RNA, 5 S RNA and proteins from Escherichia coli 50 S ribosomal subunits. FEBS Lett. 1971 Oct 15;18(1):145–148. doi: 10.1016/0014-5793(71)80431-3. [DOI] [PubMed] [Google Scholar]
- Herr W., Noller H. F. A fragment of 23S RNA containing a nucleotide sequence complementary to a region of 5S RNA. FEBS Lett. 1975 May 1;53(2):248–252. doi: 10.1016/0014-5793(75)80030-5. [DOI] [PubMed] [Google Scholar]
- Hindennach I., Kaltschmidt E., Wittmann H. G. Ribosomal proteins. Isolation of proteins from 50S ribosomal subunits of Escherichia coli. Eur J Biochem. 1971 Nov 11;23(1):12–16. doi: 10.1111/j.1432-1033.1971.tb01585.x. [DOI] [PubMed] [Google Scholar]
- Horne J. R., Erdmann V. A. Isolation and characterization of 5S RNA-protein complexes from Bacillus stearothermophilus and Escherichia coli ribosomes. Mol Gen Genet. 1972;119(4):337–344. doi: 10.1007/BF00272091. [DOI] [PubMed] [Google Scholar]
- Morrison C. A., Bradbury E. M., Garrett R. A. A comparison of the structures of several acid-urea extracted ribosomal proteins from Escherichia coli using proton NMR. FEBS Lett. 1977 Sep 15;81(2):435–439. doi: 10.1016/0014-5793(77)80572-3. [DOI] [PubMed] [Google Scholar]
- Nazar R. N., Yaguchi M., Willick G. E., Rollin C. F., Roy C. The 5-S RNA binding protein from yeast (Saccharomyces cerevisiae) ribosomes. Evolution of the eukaryotic 5-S RNA binding protein. Eur J Biochem. 1979 Dec 17;102(2):573–582. doi: 10.1111/j.1432-1033.1979.tb04274.x. [DOI] [PubMed] [Google Scholar]
- Newberry V., Brosius J., Garrett R. Fragment of protein L18 from the Escherichia coli ribosome that contains the 5S RNA binding site. Nucleic Acids Res. 1978 Jun;5(6):1753–1766. doi: 10.1093/nar/5.6.1753. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spierer P., Bogdanov A. A., Zimmermann R. A. Parameters for the interaction of ribosomal proteins L5, L18, and L25 with 5S RNA from Escherichia coli. Biochemistry. 1978 Dec 12;17(25):5394–5398. doi: 10.1021/bi00618a012. [DOI] [PubMed] [Google Scholar]
- Spierer P., Wang C. C., Marsh T. L., Zimmermann R. A. Cooperative interactions among protein and RNA components of the 50S ribosomal subunit of Escherichia coli. Nucleic Acids Res. 1979 Apr;6(4):1669–1682. doi: 10.1093/nar/6.4.1669. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yu R. S., Wittmann H. G. The sequence of steps in the attachment of 5-S RNA to cores of Escherichia coli ribosomes. Biochim Biophys Acta. 1973 Oct 26;324(3):375–385. doi: 10.1016/0005-2787(73)90282-7. [DOI] [PubMed] [Google Scholar]
- Zimmermann R. A., Stöffler G. Purification of proteins from the 50S ribosomal subunit of Escherichia coli by ion-exchange chromatography. Biochemistry. 1976 May 4;15(9):2007–2017. doi: 10.1021/bi00654a031. [DOI] [PubMed] [Google Scholar]