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. 1980 Oct 10;8(19):4337–4347. doi: 10.1093/nar/8.19.4337

Expression of large plasmids in the endosymbiotic form of Rhizobium leguminosarum.

A J Krol, J G Hontelez, R C Van den Bos, A Van Kammen
PMCID: PMC324243  PMID: 7001365

Abstract

Isolated plasmid DNA from Rhizobium leguminosarum was hybridised with cellular RNA from broth-cultured bacteria and endosymbiotic bacteroids. From these hybridisation, experiments it is concluded that plasmid genes are strongly expressed in bacteroids and only weakly or not at all in bacteria. From the hybridisation of plasmid DNA with the cloned structural nif genes of Klebsiella pneumoniae it is shown that at least part of the nif genes are located on a plasmid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bechet M., Guillaume J. B. Mise en évidence d'ADN extrachromosomique chez Rhizobium meliloti. Can J Microbiol. 1978 Aug;24(8):960–966. [PubMed] [Google Scholar]
  2. Bisseling T., van den Bos R. C., van Kammen A. The effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of leghemoglobin in pea root nodules induced by Rhizobium leguminosarum. Biochim Biophys Acta. 1978 Feb 13;539(1):1–11. doi: 10.1016/0304-4165(78)90115-0. [DOI] [PubMed] [Google Scholar]
  3. Cannon F. C., Riedel G. E., Ausubel F. M. Overlapping sequences of Klebsiella pneumoniae nifDNA cloned and characterized. Mol Gen Genet. 1979 Jul 2;174(1):59–66. doi: 10.1007/BF00433306. [DOI] [PubMed] [Google Scholar]
  4. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  5. Goldbach R. W., Borst P., Bollen-de Boer J. E., van Bruggen E. F. The organization of ribosomal RNA genes in the mitochondrial DNA of Tetrahymena pyriformis strain ST. Biochim Biophys Acta. 1978 Nov 21;521(1):169–186. doi: 10.1016/0005-2787(78)90260-5. [DOI] [PubMed] [Google Scholar]
  6. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hansen J. B., Olsen R. H. IncP2 group of Pseudomonas, a class of uniquely large plasmids. Nature. 1978 Aug 17;274(5672):715–717. doi: 10.1038/274715a0. [DOI] [PubMed] [Google Scholar]
  8. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  9. Ruvkun G. B., Ausubel F. M. Interspecies homology of nitrogenase genes. Proc Natl Acad Sci U S A. 1980 Jan;77(1):191–195. doi: 10.1073/pnas.77.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sanders J. P., Borst P., Weijers P. J. The organization of genes in yeast mitochondrial DNA II. The physical map of EcoRI and HindII + III fragments. Mol Gen Genet. 1975 Dec 30;143(1):53–64. doi: 10.1007/BF00269420. [DOI] [PubMed] [Google Scholar]
  11. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  12. Thuring R. W., Sanders J. P., Borst P. A freeze-squeeze method for recovering long DNA from agarose gels. Anal Biochem. 1975 May 26;66(1):213–220. doi: 10.1016/0003-2697(75)90739-3. [DOI] [PubMed] [Google Scholar]

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