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. 1980 Oct 10;8(19):4521–4534. doi: 10.1093/nar/8.19.4521

Chemical modification studies and the secondary structure of HeLa cell 5.8S rRNA.

J M Kelly, B E Maden
PMCID: PMC324256  PMID: 7433113

Abstract

Various secondary structure models have been proposed for 5.8 S rRNA. In this paper HeLa cell 5.8 S rRNA is shown to possess several sites that are reactive to carbodiimide at 25 degrees, and other regions that are unreactive. Previous work has established the distribution of reactive and unreactive cytidine residues along the primary structure (11). The secondary structure model of Nazar et al. (7) is fully compatible with the chemical reactivity data whereas other models are partly incompatible. We conclude that the model of Nazar et al. provides the best approximation so far available to the conformation of isolated 5.8 S rRNA. Findings on the effect temperature on the chemical reactivity of different parts of the structure are summarized. The findings described in this paper should provide a basis for examining the specific interaction of 5.8 S rRNA with 28 s rRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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