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. 1980 Nov 11;8(21):4919–4926. doi: 10.1093/nar/8.21.4919

The structure of the yeast ribosomal RNA genes. 2. The nucleotide sequence of the initiation site for ribosomal RNA transcription.

A A Bayev, O I Georgiev, A A Hadjiolov, M B Kermekchiev, N Nikolaev, K G Skryabin, V M Zakharyev
PMCID: PMC324269  PMID: 7003545

Abstract

The 5'-terminal coding sequence for the 37 S precursor to rRNA of Saccharomyces cerevisiae is identified by reverse transcriptase extension and protection mapping with nuclease S1. The sequence of a 419 bp rDNA fragment containing the transcription initiation site and its adjacent region is determined.

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Selected References

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  1. Batts-Young B., Lodish H. F. Triphosphate residues at the 5' ends of rRNA precursor and 5S RNA from Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1978 Feb;75(2):740–744. doi: 10.1073/pnas.75.2.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benoist C., O'Hare K., Breathnach R., Chambon P. The ovalbumin gene-sequence of putative control regions. Nucleic Acids Res. 1980 Jan 11;8(1):127–142. doi: 10.1093/nar/8.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  4. Boseley P., Moss T., Mächler M., Portmann R., Birnstiel M. Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis. Cell. 1979 May;17(1):19–31. doi: 10.1016/0092-8674(79)90291-5. [DOI] [PubMed] [Google Scholar]
  5. Dudov K. P., Dabeva M. D., Hadjiolov A. A. Simple agar--urea-gel electrophoretic fractionation of high molecular weight ribonucleic acids. Anal Biochem. 1976 Nov;76(50):250–258. doi: 10.1016/0003-2697(76)90283-9. [DOI] [PubMed] [Google Scholar]
  6. Glynn I. M., Chappell J. B. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. doi: 10.1042/bj0900147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hadjiolov A. A., Nikolaev N. Maturation of ribosomal ribonucleic acids and the biogenesis of ribosomes. Prog Biophys Mol Biol. 1976;31(2):95–144. doi: 10.1016/0079-6107(78)90006-8. [DOI] [PubMed] [Google Scholar]
  8. Levis R., Penman S. Processing steps and methylation in the formation of the ribosomal RNA of cultured Drosophila cells. J Mol Biol. 1978 May 15;121(2):219–238. doi: 10.1016/s0022-2836(78)80006-0. [DOI] [PubMed] [Google Scholar]
  9. Levy S., Sures I., Kedes L. H. Sequence of the 5'-end of Strongylocentrotus purpuratus H2b histone mRNA and its location within histone DNA. Nature. 1979 Jun 21;279(5715):737–739. doi: 10.1038/279737a0. [DOI] [PubMed] [Google Scholar]
  10. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maxam A. M., Tizard R., Skryabin K. G., Gilbert W. Promotor region for yeast 5S ribosomal RNA. Nature. 1977 Jun 16;267(5612):643–645. doi: 10.1038/267643a0. [DOI] [PubMed] [Google Scholar]
  12. Moss T., Birnstiel M. L. The putative promoter of a Xenopus laevis ribosomal gene is reduplicated. Nucleic Acids Res. 1979 Aug 24;6(12):3733–3743. doi: 10.1093/nar/6.12.3733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nikolaev N., Georgiev O. I., Venkov P. V., Hadjiolov A. A. The 37 S precursor to ribosomal RNA is the primary transcript of ribosomal RNA genes in Saccharomyces cerevisiae. J Mol Biol. 1979 Jan 25;127(3):297–308. doi: 10.1016/0022-2836(79)90331-0. [DOI] [PubMed] [Google Scholar]
  14. Niles E. G. Isolation of a high specific activity 35S ribosomal RNA precursor from Tetrahymena pyriformis and identification of its 5' terminus, pppAp. Biochemistry. 1978 Oct 31;17(22):4839–4844. doi: 10.1021/bi00615a035. [DOI] [PubMed] [Google Scholar]
  15. Petes T. D., Hereford L. M., Skryabin K. G. Characterization of two types of yeast ribosomal DNA genes. J Bacteriol. 1978 Apr;134(1):295–305. doi: 10.1128/jb.134.1.295-305.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reeder R. H., Sollner-Webb B., Wahn H. L. Sites of transcription initiation in vivo on Xenopus laevis ribosomal DNA. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5402–5406. doi: 10.1073/pnas.74.12.5402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. doi: 10.1146/annurev.ge.13.120179.001535. [DOI] [PubMed] [Google Scholar]
  18. Rungger D., Crippa M. The primary ribosomal DNA transcript in eukaryotes. Prog Biophys Mol Biol. 1977;31(3):247–269. doi: 10.1016/0079-6107(78)90010-x. [DOI] [PubMed] [Google Scholar]
  19. Skriabin K. G., Zakhar'ev V. M., Rubtsov P. M., Baev A. A. Posledovatel'nost' nukleotidov predpolagaemoi oblasti initsiatsii transkriptsii ribosomnogo operona drozhzhei. Dokl Akad Nauk SSSR. 1979;247(5):1275–1277. [PubMed] [Google Scholar]
  20. Sollner-Webb B., Reeder R. H. The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis. Cell. 1979 Oct;18(2):485–499. doi: 10.1016/0092-8674(79)90066-7. [DOI] [PubMed] [Google Scholar]
  21. Tanaka T., Weisblum B. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol. 1975 Jan;121(1):354–362. doi: 10.1128/jb.121.1.354-362.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Valenzuela P., Bell G. I., Masiarz F. R., DeGennaro L. J., Rutter W. J. Nucleotide sequence of the yeast 5S ribosomal RNA gene and adjacent putative control regions. Nature. 1977 Jun 16;267(5612):641–643. doi: 10.1038/267641a0. [DOI] [PubMed] [Google Scholar]
  23. Valenzuela P., Bell G. I., Venegas A., Sewell E. T., Masiarz F. R., DeGennaro L. J., Weinberg F., Rutter W. J. Ribosomal RNA genes of Saccharomyces cerevisiae. II. Physical map and nucleotide sequence of the 5 S ribosomal RNA gene and adjacent intergenic regions. J Biol Chem. 1977 Nov 25;252(22):8126–8135. [PubMed] [Google Scholar]
  24. Venkov P. V., Hadjiolov A. A., Battaner E., Schlessinger D. Saccharomyces cerevisiae: sorbitol-dependent fragile mutants. Biochem Biophys Res Commun. 1974 Feb 4;56(3):599–604. doi: 10.1016/0006-291x(74)90646-9. [DOI] [PubMed] [Google Scholar]
  25. Venkov P. V. Low ribonuclease activity in cellular lysates of osmotic sensitive Saccharomyces cerevisiae mutants. Mol Gen Genet. 1979 Aug;175(1):111–112. doi: 10.1007/BF00267862. [DOI] [PubMed] [Google Scholar]

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