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. 1980 Nov 25;8(22):5169–5178. doi: 10.1093/nar/8.22.5169

Nonsense suppression in eukaryotes: the use of the Xenopus oocyte as an in vivo assay system.

M Bienz, E Kubli, J Kohli, S de Henau, H Grosjean
PMCID: PMC324292  PMID: 7465411

Abstract

Amber, ochre, and opal nonsense suppressor tRNAs isolated from yeast were injected into Xenopus laevis oocytes together with purified mRNAs (globin mRNA from rabbit, tobacco mosaic virus-RNA). Yeast opal suppressor tRNA is able to read the UGA stop codon of the rabbit beta-globin mRNA, thus producing a readthrough protein. A large readthrough product is also obtained upon coinjection of yeast amber or ochre suppressor tRNA with TMV-RNA. The amount of readthrough product is dependent on the amount of injected suppressor tRNA. The suppression of the terminator codon of TMV-RNA is not susceptible to Mg++ concentration or polyamine addition. Therefore, the Xenopus laevis oocyte provides a simple, sensitive, and well buffered in vivo screening system for all three types of eukaryotic nonsense suppressor tRNAs.

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Selected References

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