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. 1980 Nov 25;8(22):5347–5361. doi: 10.1093/nar/8.22.5347

Mobile dispersed genetic element MDG1 of Drosophila melanogaster: transcription pattern.

Y Ilyin, V G Chmeliauskaite, V V Kulguskin, G P Georgiev
PMCID: PMC324306  PMID: 6162150

Abstract

Virtually the whole region of the mobile dispersed genetic element mdg1 including the direct repeats that frame mdg1 /1/ is transcribed. The end of the transcriptional unit is located within the direct repeat. Although the transcription of mdg1 is symmetric, one direction is transcribed preferentially and is a full-length transcript represented by approximately 29S poly(A)+RNA. Besides it, approximately 15S poly(A)+RNA is present in the cytoplasm of culture cells which corresponds to the 3'-part of 29S RNA and possibly to a small region near the 5'-end of the latter. The major fraction of mdg1 transcripts is present in the fraction of free cytoplasmic RNP particles while only 10-20% are recovered in polysomes. In this respect, mdg1 is similar to mdg3 and quite different from two single-copy genes transcripts of which are almost completely recovered in polysomes. The relationship between mobile dispersed genetic elements and endogeneous viral genomes is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Canaani E., Aaronson S. A. Restriction enzyme analysis of mouse cellular type C viral DNA: emergence of new viral sequences in spontaneous AKR/J lymphomas. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1677–1681. doi: 10.1073/pnas.76.4.1677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Finnegan D. J., Rubin G. M., Young M. W., Hogness D. S. Repeated gene families in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1053–1063. doi: 10.1101/sqb.1978.042.01.106. [DOI] [PubMed] [Google Scholar]
  3. Gehring W. J., Paro R. Isolation of a hybrid plasmid with homologous sequences to a transposing element of Drosophila melanogaster. Cell. 1980 Apr;19(4):897–904. doi: 10.1016/0092-8674(80)90081-1. [DOI] [PubMed] [Google Scholar]
  4. Gilboa E., Mitra S. W., Goff S., Baltimore D. A detailed model of reverse transcription and tests of crucial aspects. Cell. 1979 Sep;18(1):93–100. doi: 10.1016/0092-8674(79)90357-x. [DOI] [PubMed] [Google Scholar]
  5. Ilyin Y. V., Chmeliauskaite V. G., Ananiev E. V., Lyubomirskaya N. V., Kulguskin V. V., Bayev A. A., Jr, Georgiev G. P. Mobile dispersed genetic element MDG1 of Drosophila melanogaster: structural organization. Nucleic Acids Res. 1980 Nov 25;8(22):5333–5346. doi: 10.1093/nar/8.22.5333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ilyin Y. V., Chmeliauskaite V. G., Georgiev G. P. Double-stranded sequences in RNA of Drosophila melanogaster: relation to mobile dispersed genes. Nucleic Acids Res. 1980 Aug 11;8(15):3439–3457. doi: 10.1093/nar/8.15.3439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McKenzie S. L., Henikoff S., Meselson M. Localization of RNA from heat-induced polysomes at puff sites in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1117–1121. doi: 10.1073/pnas.72.3.1117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mellon P., Duesberg P. H. Subgenomic, cellular Rous sarcoma virus RNAs contain oligonucleotides from the 3' half and the 5' terminus of virion RNA. Nature. 1977 Dec 15;270(5638):631–634. doi: 10.1038/270631a0. [DOI] [PubMed] [Google Scholar]
  9. Rothenberg E., Donoghue D. J., Baltimore D. Analysis of a 5' leader sequence on murine leukemia virus 21S RNA: heteroduplex mapping with long reverse transcriptase products. Cell. 1978 Mar;13(3):435–451. doi: 10.1016/0092-8674(78)90318-5. [DOI] [PubMed] [Google Scholar]
  10. Tchurikov N. A., Ilyin Y. V., Ananiev E. V., Georgiev G. P. The properties of gene Dm 225, a representative of dispersed repetitive genes in Drosophila melanogaster. Nucleic Acids Res. 1978 Jun;5(6):2169–2187. doi: 10.1093/nar/5.6.2169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tchurikov N. A., Zelentsova E. S., Georgiev G. P. Clusters containing different mobile dispersed genes in the genome of Drosophila melanogaster. Nucleic Acids Res. 1980 Mar 25;8(6):1243–1258. doi: 10.1093/nar/8.6.1243. [DOI] [PMC free article] [PubMed] [Google Scholar]

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