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. 1980 Dec 11;8(23):5635–5647. doi: 10.1093/nar/8.23.5635

Nucleotide sequences at the 5'-termini of the alfalfa mosaic virus RNAs and the intercistronic junction in RNA 3.

E C Koper-Zwarthoff, F T Brederode, G Veeneman, J H van Boom, J F Bol
PMCID: PMC324331  PMID: 6927843

Abstract

Nucleotide sequences at the 5'-termini of the alfalfa mosaic virus genomic RNAs and the intercistronic junction in RNA 3 were deduced and compared to identify possible common recognition signals for replicating enzymes in the corresponding minus-stranded viral RNAs. Homology between the 5'-terminal sequences is less than 11 nucleotides and no complementarity with the homologous sequence occurring at the 3'-end of the viral RNAs was observed. Homology between the 5'-terminus and intercistronic region in RNA 3 is compatible with the synthesis of subgenomic RNA 4 by internal initiation of transcription on the RNA 3 minus strands. The sequence around the intercistronic junction can be folded into a very stable secondary structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahlquist P., Dasgupta R., Shih D. S., Zimmern D., Kaesberg P. Two-step binding of eukaryotic ribosomes to brome mosaic virus RNA3. Nature. 1979 Sep 27;281(5729):277–282. doi: 10.1038/281277a0. [DOI] [PubMed] [Google Scholar]
  2. Bol J. F., Brederode F. T., Janze G. C., Rauh D. K. Studies on sequence homology between the RNA's of alfalfa mosaic virus. Virology. 1975 May;65(1):1–15. doi: 10.1016/0042-6822(75)90002-1. [DOI] [PubMed] [Google Scholar]
  3. Bol J. F., Lak-Kaashoek M. Composition of alfalfa mosaic virus nucleoproteins. Virology. 1974 Aug;60(2):476–484. doi: 10.1016/0042-6822(74)90341-9. [DOI] [PubMed] [Google Scholar]
  4. Brederode F. T., Koper-Zwarthoff E. C., Bol J. F. Complete nucleotide sequence of alfalfa mosaic virus RNA 4. Nucleic Acids Res. 1980 May 24;8(10):2213–2223. doi: 10.1093/nar/8.10.2213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Briand J. P., Keith G., Guilley H. Nucleotide sequence at the 5' extremity of turnip yellow mosaic virus genome RNA. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3168–3172. doi: 10.1073/pnas.75.7.3168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dasgupta R., Shih D. S., Saris C., Kaesberg P. Nucleotide sequence of a viral RNA fragment that binds to eukaryotic ribosomes. Nature. 1975 Aug 21;256(5519):624–628. doi: 10.1038/256624a0. [DOI] [PubMed] [Google Scholar]
  7. Donis-Keller H. Phy M: an RNase activity specific for U and A residues useful in RNA sequence analysis. Nucleic Acids Res. 1980 Jul 25;8(14):3133–3142. doi: 10.1093/nar/8.14.3133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Filipowicz W., Haenni A. L. Binding of ribosomes to 5'-terminal leader sequences of eukaryotic messenger RNAs. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3111–3115. doi: 10.1073/pnas.76.7.3111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Guilley H., Jonard G., Kukla B., Richards K. E. Sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA. Nucleic Acids Res. 1979 Apr;6(4):1287–1308. doi: 10.1093/nar/6.4.1287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koper-Zwarthoff E. C., Bol J. F. 3'-Terminal nucleotide sequence of alfalfa mosaic virus RNA 4. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1114–1117. doi: 10.1073/pnas.76.3.1114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Koper-Zwarthoff E. C., Bol J. F. Nucleotide sequence of the putative recognition site for coat protein in the RNAs of alfalfa mosaic virus and tobacco streak virus. Nucleic Acids Res. 1980 Aug 11;8(15):3307–3318. doi: 10.1093/nar/8.15.3307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Koper-Zwarthoff E. C., Brederode F. T., Walstra P., Bol J. F. Nucleotide sequence of the 3'-noncoding region of alfalfa mosaic virus RNA 4 and its homology with the genomic RNAs. Nucleic Acids Res. 1979 Dec 11;7(7):1887–1900. doi: 10.1093/nar/7.7.1887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Koper-Zwarthoff E. C., Lockard R. E., Alzner-deWeerd B., RajBhandary U. L., Bol J. F. Nucleotide sequence of 5' terminus of alfalfa mosaic virus RNA 4 leading into coat protein cistron. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5504–5508. doi: 10.1073/pnas.74.12.5504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kozak M. How do eucaryotic ribosomes select initiation regions in messenger RNA? Cell. 1978 Dec;15(4):1109–1123. doi: 10.1016/0092-8674(78)90039-9. [DOI] [PubMed] [Google Scholar]
  15. Moss B. Utilization of the guanylyltransferase and methyltransferases of vaccinia virus to modify and identify the 5'-terminals of heterologous RNA species. Biochem Biophys Res Commun. 1977 Jan 24;74(2):374–383. doi: 10.1016/0006-291x(77)90314-x. [DOI] [PubMed] [Google Scholar]
  16. Pinck L. The 5'-end groups of alfalfa mosaic virus RNAs are m7G5'ppp5'Gp. FEBS Lett. 1975 Nov 1;59(1):24–28. doi: 10.1016/0014-5793(75)80332-2. [DOI] [PubMed] [Google Scholar]
  17. Richards K., Guilley H., Jonard G., Hirth L. Nucleotide sequence at the 5' extremity of tobacco-mosaic-virus RNA. 1. The noncoding region (nucleotides 1-68). Eur J Biochem. 1978 Mar 15;84(2):513–519. doi: 10.1111/j.1432-1033.1978.tb12194.x. [DOI] [PubMed] [Google Scholar]
  18. Tinoco I., Jr, Borer P. N., Dengler B., Levin M. D., Uhlenbeck O. C., Crothers D. M., Bralla J. Improved estimation of secondary structure in ribonucleic acids. Nat New Biol. 1973 Nov 14;246(150):40–41. doi: 10.1038/newbio246040a0. [DOI] [PubMed] [Google Scholar]

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