Abstract
Physarum polycephalum, an acellular slime mold, produces an endoribonuclease activity (Phy M) useful in direct RNA sequence determination. Under conditions previously described for direct enzymatic RNA sequencing (7M urea, 50 degrees C, pH 5.0)1 Phy M cleaves almost exclusively and uniformly at Up-N and Ap-N. In the absence of urea Up-N, Ap-N, and Gp-N are attacked in a sequence specific way identical to that found with RNase Phy I2 digests performed under the same reaction conditions.
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- Adler P. N., Holt C. E. Genetic analysis in the colonia strain of Physarum polycephalum: heterothallic strains that mate with and are partially isogenic to the colonia strain. Genetics. 1974 Dec;78(4):1051–1062. doi: 10.1093/genetics/78.4.1051. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Braun R., Behrens K. A ribonuclease from Physarum. Biochemical properties and synthesis in the mitotic cycle. Biochim Biophys Acta. 1969 Nov 19;195(1):87–98. doi: 10.1016/0005-2787(69)90605-4. [DOI] [PubMed] [Google Scholar]
- Contreras R., Fiers W. A method for the isolation of cytidylate series from ribonuclease T1-oligonucleotides. Anal Biochem. 1975 Jul;67(1):319–326. doi: 10.1016/0003-2697(75)90299-7. [DOI] [PubMed] [Google Scholar]
- DANIEL J. W., RUSCH H. P. The pure culture of Physarum polycephalum on a partially defined soluble medium. J Gen Microbiol. 1961 May;25:47–59. doi: 10.1099/00221287-25-1-47. [DOI] [PubMed] [Google Scholar]
- Lockard R. E., Alzner-Deweerd B., Heckman J. E., MacGee J., Tabor M. W., RajBhandary U. L. Sequence analysis of 5'[32P] labeled mRNA and tRNA using polyacrylamide gel electrophoresis. Nucleic Acids Res. 1978 Jan;5(1):37–56. doi: 10.1093/nar/5.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pilly D., Niemeyer A., Schmidt M., Bargetzi J. P. Enzymes for RNA sequence analysis. Preparation and specificity of exoplasmodial ribonucleases I and II from Physarum polycephalum. J Biol Chem. 1978 Jan 25;253(2):437–445. [PubMed] [Google Scholar]
- Rubin G. M. Preparation of RNA and ribosomes from yeast. Methods Cell Biol. 1975;12:45–64. doi: 10.1016/s0091-679x(08)60951-6. [DOI] [PubMed] [Google Scholar]
- Rubin G. M. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid. J Biol Chem. 1973 Jun 10;248(11):3860–3875. [PubMed] [Google Scholar]
- Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]