Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1980 Jul 25;8(14):3215–3227. doi: 10.1093/nar/8.14.3215

Amplification of single-strand DNA binding protein in Escherichia coli.

J W Chase, R F Whittier, J Auerbach, A Sancar, W D Rupp
PMCID: PMC324365  PMID: 6449689

Abstract

An E. coli strain containing a recombinant plasmid carrying the E. coli ssbA+ gene has been shown to produce 12 to 15 fold increased amounts of single-strand DNA binding-protein relative to wild-type strains. In addition, a gamma transducing phage carrying the E. coli uvrA+ gene has been shown to also carry the ssbA+ gene and to be capable of producing increased amounts of binding protein.

Full text

PDF
3215

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberts B. M., Frey L. T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA. Nature. 1970 Sep 26;227(5265):1313–1318. doi: 10.1038/2271313a0. [DOI] [PubMed] [Google Scholar]
  2. Amemiya K., Wu C. W., Shapiro L. Caulobacter crescentus RNA polymerase. Purification and characterization of holoenzyme and core polymerase. J Biol Chem. 1977 Jun 25;252(12):4157–4165. [PubMed] [Google Scholar]
  3. Auerbach J., Howard-Flanders P. The isolation and genetic characteristics of lambda transducing phages of the uvrA+ and uvrC+ genes of E. coli K12. Mol Gen Genet. 1979 Jan 11;168(3):341–344. doi: 10.1007/BF00271506. [DOI] [PubMed] [Google Scholar]
  4. Dunn J. J. RNase III cleavage of single-stranded RNA. Effect of ionic strength on the fideltiy of cleavage. J Biol Chem. 1976 Jun 25;251(12):3807–3814. [PubMed] [Google Scholar]
  5. Glassberg J., Meyer R. R., Kornberg A. Mutant single-strand binding protein of Escherichia coli: genetic and physiological characterization. J Bacteriol. 1979 Oct;140(1):14–19. doi: 10.1128/jb.140.1.14-19.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gold L., O'Farrell P. Z., Russel M. Regulation of gene 32 expression during bacteriophage T4 infection of Escherichia coli. J Biol Chem. 1976 Nov 25;251(22):7251–7262. [PubMed] [Google Scholar]
  7. Hinkle D. C., Chamberlin M. J. Studies of the binding of Escherichia coli RNA polymerase to DNA. I. The role of sigma subunit in site selection. J Mol Biol. 1972 Sep 28;70(2):157–185. doi: 10.1016/0022-2836(72)90531-1. [DOI] [PubMed] [Google Scholar]
  8. Horwitz M. S. Temperature-sensitive replication of H5ts125 adenovirus DNA in vitro. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4291–4295. doi: 10.1073/pnas.75.9.4291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnson B. F. Genetic mapping of the lexC-113 mutation. Mol Gen Genet. 1977 Nov 29;157(1):91–97. doi: 10.1007/BF00268691. [DOI] [PubMed] [Google Scholar]
  10. Kunkel T. A., Meyer R. R., Loeb L. A. Single-strand binding protein enhances fidelity of DNA synthesis in vitro. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6331–6335. doi: 10.1073/pnas.76.12.6331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Meyer R. R., Glassberg J., Kornberg A. An Escherichia coli mutant defective in single-strand binding protein is defective in DNA replication. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1702–1705. doi: 10.1073/pnas.76.4.1702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Molineux I. J., Friedman S., Gefter M. L. Purification and properties of the Escherichia coli deoxyribonucleic acid-unwinding protein. Effects on deoxyribonucleic acid synthesis in vitro. J Biol Chem. 1974 Oct 10;249(19):6090–6098. [PubMed] [Google Scholar]
  15. Ray R. K., Reuben R., Molineux I., Gefter M. The purification of exonuclease I from Escherichia coli by affinity chromatography. J Biol Chem. 1974 Sep 10;249(17):5379–5381. [PubMed] [Google Scholar]
  16. Ruyechan W. T., Wetmur J. G. Studies on the cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA. Biochemistry. 1975 Dec 16;14(25):5529–5534. doi: 10.1021/bi00696a023. [DOI] [PubMed] [Google Scholar]
  17. Sancar A., Rupp W. D. Cloning of uvrA, lexC and ssb genes of Escherichia coli. Biochem Biophys Res Commun. 1979 Sep 12;90(1):123–129. doi: 10.1016/0006-291x(79)91598-5. [DOI] [PubMed] [Google Scholar]
  18. Tomizawa J. I., Anraku N., Iwama Y. Molecular mechanisms of genetic recombination in bacteriophage. VI. A mutant defective in the joining of DNA molecules. J Mol Biol. 1966 Nov 14;21(2):247–253. doi: 10.1016/0022-2836(66)90095-7. [DOI] [PubMed] [Google Scholar]
  19. Wickner S. H. DNA replication proteins of Escherichia coli. Annu Rev Biochem. 1978;47:1163–1191. doi: 10.1146/annurev.bi.47.070178.005503. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES