Figure 4.

Toll-like receptor (TLR) signalling involved in the inhibition of Gas6 and ProS expression. (a) Macrophages were treated with lipopolysaccharide (LPS) alone or with LPS and the respective inhibitors [10 μm BAY11-7082 (BAY) for p65, 50 μm SP600125 (SP) for interferon-regulating factor-3 (IRF3), 10 μm SB202190 (SB) for p38] for 8 hr. (b) Macrophages were treated with poly(I:C) alone or with poly(I:C) and the respective inhibitors for 8 hr. (c) Macrophages were treated with CpG alone or with CpG and the respective inhibitors for 8 hr. The relative mRNA levels were determined using quantitative PCR. Data represent the mean ± SEM of three experiments (n = 3 mice per experiment). **P < 0·01.