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Real-time reverse transcriptase–polymerase chain reaction quantification of seven miRs in 60 cases of thyroid fine needle aspiration specimens. Delta Ct was calculated as follows: delta Ct=Ct of the tested miR minus Ct of U6. Samples were divided into two groups: benign lesions (n=16) and malignant lesions (n=44). miRs, microRNAs.
