Table 1.

Primers used for bisulfite genomic sequencing, MSPCR, and real-time PCR

Primers for BSPCR
rBS-NSBP1	Forward	5′-GTTTTTTTGTTAATTGGGAAAGATT-3′
	Reverse	5′-AAAAAAATCTCTACCTAACTACTCC-3′
rBS-PDE4D4	Forward	5′-TTATTGTTGTGAAGAGTAGATTTTGTG-3′
	Reverse	5′-ATCCTAAATTTCTTCAAACCTAACC-3′
rBS-HPCAL1	Forward	5′-GGAGTGATTAGGTTGTAGTTGAGG-3′
	Reverse	5′-TCCTACCCTCAAATTCCTAAACTAC-3′
rBS-DNMT3b	Forward	5′-AGGTGGAGAAGTTTGGGTATTATTT-3′
	Reverse	5′-CAAAAACCTCCAAAAAACCTATC-3′
rBS-MBD2	Forward	5′-GGTTATAGTTAATTTTATTTGTAATAATGT-3′
	Reverse	5′-CCAACACTAAACCCTAATTTTC-3′
Primers for MSPCR
rMS-NSBP1	Forward	5′-CGGGTTACGTTTTTCGAATC-3′
	Reverse	5′-ATCTCTACCTAACTACTCCGCGAA-3′
rUS-NSBP1	Forward	5′-GTGGGTTATGTTTTTTGAATTGG-3′
	Reverse	5′-AAAAAAATCTCTACCTAACTACTCCACAA-3′
rMS-HPCAL1	Forward	5′-AGTGGGTTTGTAGTTGATCGTAGAC-3′
	Reverse	5′-CCCCCGTAAAAAAAATATAAACG-3′
rUS-HPCAL1	Forward	5′-GTAGTGGGTTTGTAGTTGATTGTAGAT-3′
	Reverse	5′-CCCCCATAAAAAAAATATAAACACC-3′
Primers for real-time PCR
rNSBP1	Forward	5′-CTATGCCTGTGCCCTTTACAC-3′
	Reverse	5′-TTTTGGCTTCTCCATTTTCAG-3′
rPDE4D4	Forward	5′-ACGAGCAGCACCACCAGTA-3′
	Reverse	5′-AAAGACGAGGGCCAGGACAT-3′
rHPCAL1	Forward	5′-GAGATTGTGCAGGCCATCTAC-3′
	Reverse	5′-TGCCATCATTGTTTGTGTCC-3′
rRPL19	Forward	5′-GCATATGGGCATAGGGAAGA-3′
	Reverse	5′-CCATGAGAATCCGCTTGTTT-3′
rDNMT1	Forward	5′-GAGGTGGGCGACTGCGTCTC-3′
	Reverse	5′-TGTGGATGTAGGAAAGTTGCA-3′
rDNMT3a	Forward	5′-CAGAATAGCCAAGTTCAGCAAAGTGA-3′
	Reverse	5′-CTTTGCCCTGCTTTATGGAG-3′
rDNMT3b	Forward	5′-GTTAAAGAAAGTACAGACAATAACCAC-3′
	Reverse	5′-TCTGATGACTGGCACACTCC-3′
rMeCP2	Forward	5′-GTCGCTCTGCTGGAAAGTAT-3′
	Reverse	5′-TGGGCTTCTTAGGTGGTTTC-3′
rMBD1	Forward	5′-CAGCAGTCACAACCTTCCTG-3′
	Reverse	5′-GGTGCCAATCCCTCCTATCT-3′
rMBD2	Forward	5′-GTCGGCCCAGGTAGTAATGAT-3′
	Reverse	5′-GACTCGCTCTTCCTGTTTCCT-3′
rMBD3	Forward	5′-CTGAACACTGCACTGCCTGTA-3′
	Reverse	5′-GTTTCTTCTCCCAGAAAAGCTG-3′
rMBD4	Forward	5′-CCTACCGGATCTTTTGTGTCA-3′
	Reverse	5′-GATTTTCCCAAAGCCAGTCAT-3′