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. Author manuscript; available in PMC: 2013 Jan 10.
Published in final edited form as: Biochemistry. 2011 Dec 20;51(1):63–73. doi: 10.1021/bi201570a

Table 3.

Binding of PNA and GPNA (L-series) to ribosomal A-site model RNA hairpins (Figure 7).a

Entry PNA Sequence M. Tuberculosis (HRP6) H. Sapiens (HRP5)
1 PNA10 NH2-CCCTGCTT 1.2 (1.7) 0.4 (2.0)
2 GPNA10 NH2-CCCTL-ArgTL-ArgGCT 0.5 (2.9) 2.8 (5.0)
3 PNA11 NH2-CCTGCTT 0.2 (1.1) 0.04 (0.9)
4 PNA12 NH2-CTGCTT 0.2 (1.6) 0.06 (0.9)
5 GPNA12 NH2-CTL-ArgGCTT 0.4 (1.8) 0.8 (2.1)
6 GPNA13 NH2-CTL-ArgGCTL-ArgT 2.9 (8.0) 8.1 (8.8)
7 PNA13 NH2-CCCTPCTT 1.5 (1.1) NBb
8 PNA14 NH2-CCCTPexCTT 2.0 (1.1) NBb
a

Average association constants Ka × 106 M−1 in sodium acetate buffer, pH 5.5; binding order is given in parenthesis.

b

No binding, Ka < 103 M−1.