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. 1983 Jan 25;11(2):237–249. doi: 10.1093/nar/11.2.237

Nucleotide sequence of the amylase gene from Bacillus subtilis.

M Yang, A Galizzi, D Henner
PMCID: PMC325711  PMID: 6186986

Abstract

The gene coding for amylase (EC.3.2.1.1) has been isolated and sequenced from Bacillus subtilis by cloning in lambda Charon4A and pBR322. The entire coding sequence and large preceding and following regions, comprising the presumed transcriptional and translational regulatory regions, were sequenced. The coding sequence shows a large open reading frame with a translated molecular weight of 72,800 and a presumed signal sequence of approximately thirty-two amino acids. When the intact gene is present in Escherichia coli, it confers the ability to degrade starch, indicating that the gene is expressed in a functional state.

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Selected References

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