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. 1983 Feb 11;11(3):901–910. doi: 10.1093/nar/11.3.901

cDNA cloning and nucleotide sequence comparison of Chinese hamster metallothionein I and II mRNAs.

B B Griffith, R A Walters, M D Enger, C E Hildebrand, J K Griffith
PMCID: PMC325761  PMID: 6687636

Abstract

Polyadenylated RNA was extracted from a cadmium resistant Chinese hamster (CHO) cell line, enriched for metal-induced, abundant RNA sequences and cloned as double-stranded cDNA in the plasmid pBR322. Two cDNA clones, pCHMT1 and pCHMT2, encoding two Chinese hamster isometallothioneins were identified, and the nucleotide sequence of each insert was determined. The two Chinese hamster metallothioneins show nucleotide sequence homologies of 80% in the protein coding region and approximately 35% in both the 5' and 3' untranslated regions. Interestingly, an 8 nucleotide sequence (TGTAAATA) has been conserved in sequence and position in the 3' untranslated regions of each metallothionein mRNA sequenced thus far. Estimated nucleotide substitution rates derived from interspecies comparisons were used to calculate a metallothionein gene duplication time of 45 to 120 million years ago.

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Selected References

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  1. Ashburner M., Bonner J. J. The induction of gene activity in drosophilia by heat shock. Cell. 1979 Jun;17(2):241–254. doi: 10.1016/0092-8674(79)90150-8. [DOI] [PubMed] [Google Scholar]
  2. Briggs R. W., Armitage I. M. Evidence for site-selective metal binding in calf liver metallothionein. J Biol Chem. 1982 Feb 10;257(3):1259–1262. [PubMed] [Google Scholar]
  3. Brinster R. L., Chen H. Y., Warren R., Sarthy A., Palmiter R. D. Regulation of metallothionein--thymidine kinase fusion plasmids injected into mouse eggs. Nature. 1982 Mar 4;296(5852):39–42. doi: 10.1038/296039a0. [DOI] [PubMed] [Google Scholar]
  4. Day L. E., Hirst A. J., Lai E. C., Mace M., Jr, Woo S. L. 5' Domain and nucleotide sequence of an adult chicken chromosomal beta-globin gene. Biochemistry. 1981 Apr 14;20(8):2091–2098. doi: 10.1021/bi00511a005. [DOI] [PubMed] [Google Scholar]
  5. Durnam D. M., Perrin F., Gannon F., Palmiter R. D. Isolation and characterization of the mouse metallothionein-I gene. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6511–6515. doi: 10.1073/pnas.77.11.6511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Enger M. D., Rall L. B., Hildebrand C. E. Thionein gene expression in Cd++-variants of the CHO cell: correlation of thionein synthesis rates with translatable mRNA levels during induction, deinduction, and superinduction. Nucleic Acids Res. 1979 Sep 11;7(1):271–288. doi: 10.1093/nar/7.1.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Glanville N., Durnam D. M., Palmiter R. D. Structure of mouse metallothionein-I gene and its mRNA. Nature. 1981 Jul 16;292(5820):267–269. doi: 10.1038/292267a0. [DOI] [PubMed] [Google Scholar]
  8. Goad W. B., Kanehisa M. I. Pattern recognition in nucleic acid sequences. I. A general method for finding local homologies and symmetries. Nucleic Acids Res. 1982 Jan 11;10(1):247–263. doi: 10.1093/nar/10.1.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Griffith J. K., Buckingham J. M., Hanners J. L., Hildebrand C. E., Walters R. A. Plasmid-conferred tetracycline resistance confers collateral cadmium sensitivity of E. coli cells. Plasmid. 1982 Jul;8(1):86–88. doi: 10.1016/0147-619x(82)90044-0. [DOI] [PubMed] [Google Scholar]
  10. Griffith J. K., Enger M. D., Hildebrand C. E., Walters R. A. Differential induction by cadmium of a low-complexity ribonucleic acid class in cadmium-resistant and cadmium-sensitive mammalian cells. Biochemistry. 1981 Aug 4;20(16):4755–4761. doi: 10.1021/bi00519a035. [DOI] [PubMed] [Google Scholar]
  11. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ingolia T. D., Craig E. A. Primary sequence of the 5' flanking regions of the Drosophila heat shock genes in chromosome subdivision 67B. Nucleic Acids Res. 1981 Apr 10;9(7):1627–1642. doi: 10.1093/nar/9.7.1627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Karin M., Richards R. I. Human metallothionein genes: molecular cloning and sequence analysis of the mRNA. Nucleic Acids Res. 1982 May 25;10(10):3165–3173. doi: 10.1093/nar/10.10.3165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980 Dec;16(2):111–120. doi: 10.1007/BF01731581. [DOI] [PubMed] [Google Scholar]
  15. Kimura M., Ota T. On the rate of molecular evolution. J Mol Evol. 1971;1(1):1–17. doi: 10.1007/BF01659390. [DOI] [PubMed] [Google Scholar]
  16. Lerch K., Ammer D., Olafson R. W. Crab metallothionein. Primary structures of metallothioneins 1 and 2. J Biol Chem. 1982 Mar 10;257(5):2420–2426. [PubMed] [Google Scholar]
  17. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  18. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mayo K. E., Palmiter R. D. Glucocorticoid regulation of metallothionein-I mRNA synthesis in cultured mouse cells. J Biol Chem. 1981 Mar 25;256(6):2621–2624. [PubMed] [Google Scholar]
  20. Mayo K. E., Warren R., Palmiter R. D. The mouse metallothionein-I gene is transcriptionally regulated by cadmium following transfection into human or mouse cells. Cell. 1982 May;29(1):99–108. doi: 10.1016/0092-8674(82)90094-0. [DOI] [PubMed] [Google Scholar]
  21. Mbikay M., Maiti I. B., Thirion J. P. Cloning and sequencing of cDNA for mouse liver metallothionein-I. Biochem Biophys Res Commun. 1981 Dec 15;103(3):825–832. doi: 10.1016/0006-291x(81)90885-8. [DOI] [PubMed] [Google Scholar]
  22. McKeown M., Firtel R. A. Differential expression and 5' end mapping of actin genes in Dictyostelium. Cell. 1981 Jun;24(3):799–807. doi: 10.1016/0092-8674(81)90105-7. [DOI] [PubMed] [Google Scholar]
  23. Michelson A. M., Orkin S. H. The 3' untranslated regions of the duplicated human alpha-globin genes are unexpectedly divergent. Cell. 1980 Nov;22(2 Pt 2):371–377. doi: 10.1016/0092-8674(80)90347-5. [DOI] [PubMed] [Google Scholar]
  24. Olafson R. W., Loya S., Sim R. G. Physiological parameters of prokaryotic metallothionein induction. Biochem Biophys Res Commun. 1980 Aug 29;95(4):1495–1503. doi: 10.1016/s0006-291x(80)80066-0. [DOI] [PubMed] [Google Scholar]
  25. Otvos J. D., Olafson R. W., Armitage I. M. Structure of an invertebrate metallothionein from Scylla serrata. J Biol Chem. 1982 Mar 10;257(5):2427–2431. [PubMed] [Google Scholar]
  26. Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
  27. Ricciardi R. P., Miller J. S., Roberts B. E. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927–4931. doi: 10.1073/pnas.76.10.4927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Rice N. R. Change in repeated DNA in evolution. Brookhaven Symp Biol. 1972;23:44–79. [PubMed] [Google Scholar]
  29. Roychoudhury R., Jay E., Wu R. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res. 1976 Apr;3(4):863–877. doi: 10.1093/nar/3.4.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Rubin C. M., Schmid C. W. Pyrimidine-specific chemical reactions useful for DNA sequencing. Nucleic Acids Res. 1980 Oct 24;8(20):4613–4619. doi: 10.1093/nar/8.20.4613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Thayer R. E. An improved method for detecting foreign DNA in plasmids of Escherichia coli. Anal Biochem. 1979 Sep 15;98(1):60–63. doi: 10.1016/0003-2697(79)90705-x. [DOI] [PubMed] [Google Scholar]
  32. Walters R. A., Yandell P. M., Enger M. D. Comparison of complexity and diversity of polyadenylated polysomal and informosomal messenger ribonucleic acid from Chinese hamster cells. Biochemistry. 1979 Sep 18;18(19):4254–4261. doi: 10.1021/bi00586a034. [DOI] [PubMed] [Google Scholar]
  33. Wickens M. P., Buell G. N., Schimke R. T. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I. J Biol Chem. 1978 Apr 10;253(7):2483–2495. [PubMed] [Google Scholar]
  34. Winge D. R., Miklossy K. A. Differences in the polymorphic forms of metallothionein. Arch Biochem Biophys. 1982 Mar;214(1):80–88. doi: 10.1016/0003-9861(82)90010-8. [DOI] [PubMed] [Google Scholar]

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