Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1983 Feb 25;11(4):1059–1075. doi: 10.1093/nar/11.4.1059

DNA topoisomerases from rat liver: physiological variations.

M Duguet, C Lavenot, F Harper, G Mirambeau, A M De Recondo
PMCID: PMC325776  PMID: 6298730

Abstract

Besides the nicking-closing (topoisomerase I) activity, an ATP-dependent DNA topoisomerase is present in rat liver nuclei. The enzyme, partially purified, is able to catenate in vitro closed DNA circles in a magnesium-dependent, ATP-dependent, histone H1-dependent reaction, and to decatenate in vitro kinetoplast DNA networks to yield free minicircles in a magnesium-dependent and ATP-dependent reaction. It is largely similar to other eukaryotic type II topoisomerases in its requirements, and presumably belongs to this class of enzymes. Type I and type II activities were measured in rat liver nuclei as a function of regenerating time after partial hepatectomy: type I activity was not significantly changed during this process. In contrast, type II activity was considerably increased, suggesting a possible involvement of the enzyme in DNA replication.

Full text

PDF
1059

Images in this article

1065Image
on p.1065
1067Image
on p.1067
1068Image
on p.1068
1069Image
on p.1069
1070Image
on p.1070

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Attardi D. G., De Paolis A., Tocchini-Valentini G. P. Purification and characterization of Xenopus laevis type I topoisomerase. J Biol Chem. 1981 Apr 25;256(8):3654–3661. [PubMed] [Google Scholar]
  2. Baldi M. I., Benedetti P., Mattoccia E., Tocchini-Valentini G. P. In vitro catenation and decatenation of DNA and a novel eucaryotic ATP-dependent topoisomerase. Cell. 1980 Jun;20(2):461–467. doi: 10.1016/0092-8674(80)90632-7. [DOI] [PubMed] [Google Scholar]
  3. Been M. D., Champoux J. J. DNA breakage and closure by rat liver type 1 topoisomerase: separation of the half-reactions by using a single-stranded DNA substrate. Proc Natl Acad Sci U S A. 1981 May;78(5):2883–2887. doi: 10.1073/pnas.78.5.2883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blobel G., Potter V. R. Nuclei from rat liver: isolation method that combines purity with high yield. Science. 1966 Dec 30;154(3757):1662–1665. doi: 10.1126/science.154.3757.1662. [DOI] [PubMed] [Google Scholar]
  5. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  6. CAIRNS J. The bacterial chromosome and its manner of replication as seen by autoradiography. J Mol Biol. 1963 Mar;6:208–213. doi: 10.1016/s0022-2836(63)80070-4. [DOI] [PubMed] [Google Scholar]
  7. Champoux J. J. DNA is linked to the rat liver DNA nicking-closing enzyme by a phosphodiester bond to tyrosine. J Biol Chem. 1981 May 25;256(10):4805–4809. [PubMed] [Google Scholar]
  8. Champoux J. J., Dulbecco R. An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay). Proc Natl Acad Sci U S A. 1972 Jan;69(1):143–146. doi: 10.1073/pnas.69.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Champoux J. J., McConaughy B. L. Purification and characterization of the DNA untwisting enzyme from rat liver. Biochemistry. 1976 Oct 19;15(21):4638–4642. doi: 10.1021/bi00666a014. [DOI] [PubMed] [Google Scholar]
  10. Champoux J. J. Renaturation of complementary single-stranded DNA circles: complete rewinding facilitated by the DNA untwisting enzyme. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5328–5332. doi: 10.1073/pnas.74.12.5328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Champoux J. J., Young L. S., Been M. D. Studies on the regulation and specificity of the DNA-untwisting enzyme. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):53–58. doi: 10.1101/sqb.1979.043.01.009. [DOI] [PubMed] [Google Scholar]
  12. Chang L. M., Bollum F. J. Variation of deoxyribonucleic acid polymerase activities during rat liver regeneration. J Biol Chem. 1972 Dec 25;247(24):7948–7950. [PubMed] [Google Scholar]
  13. Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
  14. Cozzarelli N. R. DNA gyrase and the supercoiling of DNA. Science. 1980 Feb 29;207(4434):953–960. doi: 10.1126/science.6243420. [DOI] [PubMed] [Google Scholar]
  15. Crumplin G. C. The involvement of DNA topoisomerases in DNA repair and mutagenesis. Carcinogenesis. 1981;2(2):157–160. doi: 10.1093/carcin/2.2.157. [DOI] [PubMed] [Google Scholar]
  16. DiNardo S., Voelkel K. A., Sternglanz R., Reynolds A. E., Wright A. Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes. Cell. 1982 Nov;31(1):43–51. doi: 10.1016/0092-8674(82)90403-2. [DOI] [PubMed] [Google Scholar]
  17. Dynan W. S., Jendrisak J. J., Hager D. A., Burgess R. R. Purification and characterization of wheat germ DNA topoisomerase I (nicking-closing enzyme). J Biol Chem. 1981 Jun 10;256(11):5860–5865. [PubMed] [Google Scholar]
  18. Fiszer-Szafarz B., Szafarz D., Guevara de Murillo A. A general, fast, and sensitive micromethod for DNA determination application to rat and mouse liver, rat hepatoma, human leukocytes, chicken fibroblasts, and yeast cells. Anal Biochem. 1981 Jan 1;110(1):165–170. doi: 10.1016/0003-2697(81)90130-5. [DOI] [PubMed] [Google Scholar]
  19. Forterre P. Model for the supercoiling reaction catalyzed by DNA gyrase. J Theor Biol. 1980 Jan 21;82(2):255–269. doi: 10.1016/0022-5193(80)90102-2. [DOI] [PubMed] [Google Scholar]
  20. Fukata H., Fukasawa H. Isolation and partial characterization of two distinct DNA topoisomerases from cauliflower inflorescence. J Biochem. 1982 Apr;91(4):1337–1342. doi: 10.1093/oxfordjournals.jbchem.a133820. [DOI] [PubMed] [Google Scholar]
  21. Gellert M. DNA topoisomerases. Annu Rev Biochem. 1981;50:879–910. doi: 10.1146/annurev.bi.50.070181.004311. [DOI] [PubMed] [Google Scholar]
  22. Gellert M., Mizuuchi K., O'Dea M. H., Nash H. A. DNA gyrase: an enzyme that introduces superhelical turns into DNA. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3872–3876. doi: 10.1073/pnas.73.11.3872. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Goto T., Wang J. C. Yeast DNA topoisomerase II. An ATP-dependent type II topoisomerase that catalyzes the catenation, decatenation, unknotting, and relaxation of double-stranded DNA rings. J Biol Chem. 1982 May 25;257(10):5866–5872. [PubMed] [Google Scholar]
  24. Hays J. B., Boehmer S. Antagonists of DNA gyrase inhibit repair and recombination of UV-irradiated phage lambda. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4125–4129. doi: 10.1073/pnas.75.9.4125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Hsieh T., Brutlag D. ATP-dependent DNA topoisonmerase from D. melanogaster reversibly catenates duplex DNA rings. Cell. 1980 Aug;21(1):115–125. doi: 10.1016/0092-8674(80)90119-1. [DOI] [PubMed] [Google Scholar]
  26. Keller W. Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4876–4880. doi: 10.1073/pnas.72.12.4876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Kreuzer K. N., Cozzarelli N. R. Formation and resolution of DNA catenanes by DNA gyrase. Cell. 1980 May;20(1):245–254. doi: 10.1016/0092-8674(80)90252-4. [DOI] [PubMed] [Google Scholar]
  28. Liu L. F., Liu C. C., Alberts B. M. T4 DNA topoisomerase: a new ATP-dependent enzyme essential for initiation of T4 bacteriophage DNA replication. Nature. 1979 Oct 11;281(5731):456–461. doi: 10.1038/281456a0. [DOI] [PubMed] [Google Scholar]
  29. Liu L. F., Liu C. C., Alberts B. M. Type II DNA topoisomerases: enzymes that can unknot a topologically knotted DNA molecule via a reversible double-strand break. Cell. 1980 Mar;19(3):697–707. doi: 10.1016/s0092-8674(80)80046-8. [DOI] [PubMed] [Google Scholar]
  30. Liu L. F., Miller K. G. Eukaryotic DNA topoisomerases: two forms of type I DNA topoisomerases from HeLa cell nuclei. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3487–3491. doi: 10.1073/pnas.78.6.3487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. May E., May P., Weil R. "Early" virus-specific RNA may contain information necessary for chromosome replication and mitosis induced by Simian Virus 40. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1654–1658. doi: 10.1073/pnas.70.6.1654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Mechali M., Abadiedebat J., de Recondo A. M. Eukaryotic DNA polymerase alpha. Structural analysis of the enzyme from regenerating rat liver. J Biol Chem. 1980 Mar 10;255(5):2114–2122. [PubMed] [Google Scholar]
  33. Miller K. G., Liu L. F., Englund P. T. A homogeneous type II DNA topoisomerase from HeLa cell nuclei. J Biol Chem. 1981 Sep 10;256(17):9334–9339. [PubMed] [Google Scholar]
  34. Orr E., Fairweather N. F., Holland I. B., Pritchard R. H. Isolation and characterisation of a strain carrying a conditional lethal mutation in the cou gene of Escherichia coli K12. Mol Gen Genet. 1979;177(1):103–112. doi: 10.1007/BF00267259. [DOI] [PubMed] [Google Scholar]
  35. Prell B., Vosberg H. P. Analysis of covalent complexes formed between calf thymus DNA topoisomerase and single-stranded DNA. Eur J Biochem. 1980 Jul;108(2):389–398. doi: 10.1111/j.1432-1033.1980.tb04734.x. [DOI] [PubMed] [Google Scholar]
  36. Pruss G. J., Manes S. H., Drlica K. Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes. Cell. 1982 Nov;31(1):35–42. doi: 10.1016/0092-8674(82)90402-0. [DOI] [PubMed] [Google Scholar]
  37. Pulleyblank D. E., Ellison M. J. Purification and properties of type 1 topoisomerase from chicken erythrocytes: mechanism of eukaryotic topoisomerase action. Biochemistry. 1982 Mar 16;21(6):1155–1161. doi: 10.1021/bi00535a008. [DOI] [PubMed] [Google Scholar]
  38. Rosenberg B. H., Ungers G., Deutsch J. F. Variation in DNA swivel enzyme activity during the mammalian cell cycle. Nucleic Acids Res. 1976 Dec;3(12):3305–3311. doi: 10.1093/nar/3.12.3305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Rowe T. C., Rusche J. R., Brougham M. J., Holloman W. K. Purification and properties of a topoisomerase from Ustilago maydis. J Biol Chem. 1981 Oct 25;256(20):10354–10361. [PubMed] [Google Scholar]
  40. Sternglanz R., DiNardo S., Voelkel K. A., Nishimura Y., Hirota Y., Becherer K., Zumstein L., Wang J. C. Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition. Proc Natl Acad Sci U S A. 1981 May;78(5):2747–2751. doi: 10.1073/pnas.78.5.2747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Sundin O., Varshavsky A. Arrest of segregation leads to accumulation of highly intertwined catenated dimers: dissection of the final stages of SV40 DNA replication. Cell. 1981 Sep;25(3):659–669. doi: 10.1016/0092-8674(81)90173-2. [DOI] [PubMed] [Google Scholar]
  42. Wang J. C. Interaction between DNA and an Escherichia coli protein omega. J Mol Biol. 1971 Feb 14;55(3):523–533. doi: 10.1016/0022-2836(71)90334-2. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES