Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1983 Mar 11;11(5):1389–1404. doi: 10.1093/nar/11.5.1389

Acetylation and methylation patterns of core histones are modified after heat or arsenite treatment of Drosophila tissue culture cells

André-Patrick Arrigo 1
PMCID: PMC325804  PMID: 6402762

Abstract

Exposure of Drosophilamelanogaster tissue culture cells to 37°C (heat shock) or to arsenite induces a severe deacetylation of core histones and blocks the methylation of histone H3 and H4. Heat shock induces the methylation of histone H2b. These results are discussed in view of chromatin structure and function.

Full text

PDF
1389

Images in this article

1392Image
on p.1392
1396Image
on p.1396
1399Image
on p.1399

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLFREY V. G., FAULKNER R., MIRSKY A. E. ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS. Proc Natl Acad Sci U S A. 1964 May;51:786–794. doi: 10.1073/pnas.51.5.786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arrigo A. P., Ahmad-Zadeh C. Immunofluorescence localization of a small heat shock protein (hsp 23) in salivary gland cells of Drosophila melanogaster. Mol Gen Genet. 1981;184(1):73–79. doi: 10.1007/BF00271198. [DOI] [PubMed] [Google Scholar]
  3. Arrigo A. P., Fakan S., Tissières A. Localization of the heat shock-induced proteins in Drosophila melanogaster tissue culture cells. Dev Biol. 1980 Jul;78(1):86–103. doi: 10.1016/0012-1606(80)90320-6. [DOI] [PubMed] [Google Scholar]
  4. Arrigo A. P. Investigation of the function of the heat shock proteins in Drosophila melanogaster tissue culture cells. Mol Gen Genet. 1980;178(3):517–524. doi: 10.1007/BF00337856. [DOI] [PubMed] [Google Scholar]
  5. Bonner J. J., Pardue M. L. The effect of heat shock on RNA synthesis in Drosophila tissues. Cell. 1976 May;8(1):43–50. doi: 10.1016/0092-8674(76)90183-5. [DOI] [PubMed] [Google Scholar]
  6. Chamberlain J. P. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem. 1979 Sep 15;98(1):132–135. doi: 10.1016/0003-2697(79)90716-4. [DOI] [PubMed] [Google Scholar]
  7. Covault J., Perry M., Chalkley R. Effects of histone hyperacetylation and hypoacetylation on RNA synthesis in HTC cells. J Biol Chem. 1982 Nov 25;257(22):13433–13440. [PubMed] [Google Scholar]
  8. DiDomenico B. J., Bugaisky G. E., Lindquist S. The heat shock response is self-regulated at both the transcriptional and posttranscriptional levels. Cell. 1982 Dec;31(3 Pt 2):593–603. doi: 10.1016/0092-8674(82)90315-4. [DOI] [PubMed] [Google Scholar]
  9. Hancock R., Faber A. J., Fakan S. Isolation of interphase chromatin structures from cultured cells. Methods Cell Biol. 1977;15:127–147. doi: 10.1016/s0091-679x(08)60213-7. [DOI] [PubMed] [Google Scholar]
  10. Hancock R. Interphase chromosomal deoxyribonucleoprotein isolated as a discrete structure from cultured cells. J Mol Biol. 1974 Jul 5;86(3):649–663. doi: 10.1016/0022-2836(74)90187-9. [DOI] [PubMed] [Google Scholar]
  11. Henikoff S., Meselson M. Transcription at two heat shock loci in Drosophila. Cell. 1977 Oct;12(2):441–451. doi: 10.1016/0092-8674(77)90120-9. [DOI] [PubMed] [Google Scholar]
  12. Jackson V., Shires A., Chalkley R., Granner D. K. Studies on highly metabolically active acetylation and phosphorylation of histones. J Biol Chem. 1975 Jul 10;250(13):4856–4863. [PubMed] [Google Scholar]
  13. Johnston D., Oppermann H., Jackson J., Levinson W. Induction of four proteins in chick embryo cells by sodium arsenite. J Biol Chem. 1980 Jul 25;255(14):6975–6980. [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Lewis M., Helmsing P. J., Ashburner M. Parallel changes in puffing activity and patterns of protein synthesis in salivary glands of Drosophila. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3604–3608. doi: 10.1073/pnas.72.9.3604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McKenzie S. L., Meselson M. Translation in vitro of Drosophila heat-shock messages. J Mol Biol. 1977 Nov 25;117(1):279–283. doi: 10.1016/0022-2836(77)90035-3. [DOI] [PubMed] [Google Scholar]
  17. McKnight G. S., Hager L., Palmiter R. D. Butyrate and related inhibitors of histone deacetylation block the induction of egg white genes by steroid hormones. Cell. 1980 Nov;22(2 Pt 2):469–477. doi: 10.1016/0092-8674(80)90357-8. [DOI] [PubMed] [Google Scholar]
  18. Mirault M. E., Goldschmidt-Clermont M., Moran L., Arrigo A. P., Tissières A. The effect of heat shock on gene expression in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):819–827. doi: 10.1101/sqb.1978.042.01.082. [DOI] [PubMed] [Google Scholar]
  19. Mirault M. E., Southgate R., Delwart E. Regulation of heat-shock genes: a DNA sequence upstream of Drosophila hsp70 genes is essential for their induction in monkey cells. EMBO J. 1982;1(10):1279–1285. doi: 10.1002/j.1460-2075.1982.tb00025.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Moran L., Mirault M. E., Arrigo A. P., Goldschmidt-Clermont M., Tissières A. Heat shock of Drosophila melanogaster induces the synthesis of new messenger RNAs and proteins. Philos Trans R Soc Lond B Biol Sci. 1978 May 11;283(997):391–406. doi: 10.1098/rstb.1978.0044. [DOI] [PubMed] [Google Scholar]
  21. Osipova T. N., Vorob'ev V. I., Böttger M., von Mickwitz C. U., Scherneck S. The role of histone H2B from sea urchin sperm in the association of reconstituted minichromosomes. Mol Biol Rep. 1982 Mar 31;8(2):71–75. doi: 10.1007/BF00778507. [DOI] [PubMed] [Google Scholar]
  22. Paik W. K., Kim S. Protein methylation: chemical, enzymological, and biological significance. Adv Enzymol Relat Areas Mol Biol. 1975;42:227–286. doi: 10.1002/9780470122877.ch5. [DOI] [PubMed] [Google Scholar]
  23. Perry M., Chalkley R. Histone acetylation increases the solubility of chromatin and occurs sequentially over most of the chromatin. A novel model for the biological role of histone acetylation. J Biol Chem. 1982 Jul 10;257(13):7336–7347. [PubMed] [Google Scholar]
  24. RITOSSA F. M. BEHAVIOUR OF RNA AND DNA SYNTHESIS AT THE PUFF LEVEL IN SALIVARY GLAND CHROMOSOMES OF DROSOPHILA. Exp Cell Res. 1964 Dec;36:515–523. doi: 10.1016/0014-4827(64)90308-8. [DOI] [PubMed] [Google Scholar]
  25. Sanders L. A., Schechter N. M., McCarty K. S. A comparative study of histone acetylation, histone deacetylation, and ribonucleic acid synthesis in avian reticulocytes and erythrocytes. Biochemistry. 1973 Feb 27;12(5):783–791. doi: 10.1021/bi00729a001. [DOI] [PubMed] [Google Scholar]
  26. Sanders M. M. Identification of histone H2b as a heat-shock protein in Drosophila. J Cell Biol. 1981 Nov;91(2 Pt 1):579–583. doi: 10.1083/jcb.91.2.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Spradling A., Pardue M. L., Penman S. Messenger RNA in heat-shocked Drosophila cells. J Mol Biol. 1977 Feb 5;109(4):559–587. doi: 10.1016/s0022-2836(77)80091-0. [DOI] [PubMed] [Google Scholar]
  28. Sterner R., Vidali G., Allfrey V. G. Studies of acetylation and deacetylation in high mobility group proteins. Identification of the sites of acetylation in HMG-1. J Biol Chem. 1979 Nov 25;254(22):11577–11583. [PubMed] [Google Scholar]
  29. Tissières A., Mitchell H. K., Tracy U. M. Protein synthesis in salivary glands of Drosophila melanogaster: relation to chromosome puffs. J Mol Biol. 1974 Apr 15;84(3):389–398. doi: 10.1016/0022-2836(74)90447-1. [DOI] [PubMed] [Google Scholar]
  30. Wang C., Lazarides E., O'Connor C. M., Clarke S. Methylation of chicken fibroblast heat shock proteins at lysyl and arginyl residues. J Biol Chem. 1982 Jul 25;257(14):8356–8362. [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES