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. 1983 Mar 11;11(5):1491–1505. doi: 10.1093/nar/11.5.1491

A G43 to U43 mutation in E. coli tRNAtyrsu3+ which affects processing by RNase P.

P J Furdon, C Guerrier-Takada, S Altman
PMCID: PMC325810  PMID: 6298744

Abstract

A novel mutation in the anticodon stem of E. coli tRNA1Tyrsu3+ (G43 to U43) has been characterized. The gene coding for the mutant tRNA, carried by phage phi 80DHA61.3 a derivative of phi 80psu3+su0, produces only 20% of mature suppressor tRNA as compared with phi 80psu3+. Both the mutant tRNA precursor and mature tRNA have an altered conformation. The precursor tRNA coded for by phi 80DHA61.3 is processed by RNase P more slowly than the su3+ precursor and does not form as stable an enzyme-substrate complex as does su3+ precursor. phi 80 DHA61.3 also contains a large deletion which begins in the spacer region between the su3+ gene and the su0- gene, extends through the su0- gene and includes most of the repeated region following the tRNA genes.

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