Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1983 Mar 11;11(5):1543–1553. doi: 10.1093/nar/11.5.1543

Molecular cloning and sequencing of mRNAs coding for minor adult globin polypeptides of Xenopus laevis.

W Knöchel, W Meyerhof, S Hummel, U Grundmann
PMCID: PMC325814  PMID: 6298748

Abstract

Globin mRNA was isolated from immature red blood cells of an adult Xenopus laevis female. mRNA/cDNA hybrids were integrated in the Pst I cleavage site of pBR 322 by G/C tailing, and cloned in Escherichia coli strain HB 101. By restriction site analysis as well as hybridization behaviour we identified two clones coding for minor adult alpha and beta globin chains. Nucleotide sequence analysis and derived amino acid sequences are presented.

Full text

PDF
1543

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Battaglia P., Melli M. Isolation of globin messenger RNA of Xenopus laevis. Dev Biol. 1977 Oct 15;60(2):337–350. doi: 10.1016/0012-1606(77)90132-4. [DOI] [PubMed] [Google Scholar]
  4. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bisbee C. A., Baker M. A., Wilson A. C., Haji-Azimi I., Fischberg M. Albumin phylogeny for clawed frogs (Xenopus). Science. 1977 Feb 25;195(4280):785–787. doi: 10.1126/science.65013. [DOI] [PubMed] [Google Scholar]
  6. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hentschel C. C., Kay R. M., Williams J. G. Analysis of Xenopus laevis globins during development and erythroid cell maturation and the construction of recombinant plasmids containing sequences derived from adult globin mRNA. Dev Biol. 1979 Oct;72(2):350–363. doi: 10.1016/0012-1606(79)90124-6. [DOI] [PubMed] [Google Scholar]
  8. Hosbach H. A., Wyler T., Weber R. The Xenopus laevis globin gene family: chromosomal arrangement and gene structure. Cell. 1983 Jan;32(1):45–53. doi: 10.1016/0092-8674(83)90495-6. [DOI] [PubMed] [Google Scholar]
  9. Humphries P., Old R., Coggins L. W., McShane T., Watson C., Paul J. Recombinant plasmids containing Xenopus laevis globin structural genes derived from complementary DNA. Nucleic Acids Res. 1978 Mar;5(3):905–924. doi: 10.1093/nar/5.3.905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jeffreys A. J., Wilson V., Wood D., Simons J. P., Kay R. M., Williams J. G. Linkage of adult alpha- and beta-globin genes in X. laevis and gene duplication by tetraploidization. Cell. 1980 Sep;21(2):555–564. doi: 10.1016/0092-8674(80)90493-6. [DOI] [PubMed] [Google Scholar]
  11. Kay R. M., Harris R., Patient R. K., Williams J. G. Complete nucleotide sequence of a cloned cDNA derived from the major adult alpha-globin mRNA of X. laevis. Nucleic Acids Res. 1983 Mar 11;11(5):1537–1542. doi: 10.1093/nar/11.5.1537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kay R. M., Harris R., Patient R. K., Williams J. G. Molecular cloning of cDNA sequences coding for the major alpha- and beta-globin polypeptides of adult Xenopus laevis. Nucleic Acids Res. 1980 Jun 25;8(12):2691–2707. doi: 10.1093/nar/8.12.2691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Knöchel W., Wittig B., Wittig S., John M. E., Grundmann U., Oberthür W., Godovac J., Braunitzer G. No evidence for 'stress' alpha-globin genes in chicken. Nature. 1982 Feb 25;295(5851):710–712. doi: 10.1038/295710a0. [DOI] [PubMed] [Google Scholar]
  14. Konkel D. A., Tilghman S. M., Leder P. The sequence of the chromosomal mouse beta-globin major gene: homologies in capping, splicing and poly(A) sites. Cell. 1978 Dec;15(4):1125–1132. doi: 10.1016/0092-8674(78)90040-5. [DOI] [PubMed] [Google Scholar]
  15. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  16. Nishioka Y., Leder P. The complete sequence of a chromosomal mouse alpha--globin gene reveals elements conserved throughout vertebrate evolution. Cell. 1979 Nov;18(3):875–882. doi: 10.1016/0092-8674(79)90139-9. [DOI] [PubMed] [Google Scholar]
  17. Oberthür W., Braunitzer G., Kalas S. Hämoglobine, XLII: Untersuchungen am Hämoglobin der Graugans (Anser anser). Die Primärstruktur der alpha- und beta-Ketten der Hauptkomponents. Hoppe Seylers Z Physiol Chem. 1981 Aug;362(8):1101–1112. [PubMed] [Google Scholar]
  18. Richards R. I., Shine J., Ullrich A., Wells J. R., Goodman H. M. Molecular cloning and sequence analysis of adult chicken betal globin cDNA. Nucleic Acids Res. 1979 Nov 10;7(5):1137–1146. doi: 10.1093/nar/7.5.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Richardson C., Cappello J., Cochran M. D., Armentrout R. W., Brown R. D. Partial sequence analysis of Xenopus alpha- and beta-globin mRNA as determined from recombinant DNA plasmids. Dev Biol. 1980 Jul;78(1):161–172. doi: 10.1016/0012-1606(80)90326-7. [DOI] [PubMed] [Google Scholar]
  20. Roychoudhury R., Jay E., Wu R. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res. 1976 Jan;3(1):101–116. doi: 10.1093/nar/3.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  22. Thiébaud C. H., Fischberg M. DNA content in the genus Xenopus. Chromosoma. 1977 Feb 3;59(3):253–257. doi: 10.1007/BF00292781. [DOI] [PubMed] [Google Scholar]
  23. Thomas N., Maclean N. The erythroid cells of anaemic Xenopus laevis. I. Studies on cellular morphology and protein and nucleic acid synthesis during differentiation. J Cell Sci. 1975 Dec;19(3):509–520. doi: 10.1242/jcs.19.3.509. [DOI] [PubMed] [Google Scholar]
  24. Wensink P. C., Finnegan D. J., Donelson J. E., Hogness D. S. A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster. Cell. 1974 Dec;3(4):315–325. doi: 10.1016/0092-8674(74)90045-2. [DOI] [PubMed] [Google Scholar]
  25. Widmer H. J., Andres A. C., Niessing J., Hosbach H. A., Weber R. Comparative analysis of cloned larval and adult globin cDNA sequences of Xenopus laevis. Dev Biol. 1981 Dec;88(2):325–332. doi: 10.1016/0012-1606(81)90176-7. [DOI] [PubMed] [Google Scholar]
  26. Williams J. G., Kay R. M., Patient R. K. The nucleotide sequence of the major beta-globin mRNA from Xenopus laevis. Nucleic Acids Res. 1980 Sep 25;8(18):4247–4258. doi: 10.1093/nar/8.18.4247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Zain S., Sambrook J., Roberts R. J., Keller W., Fried M., Dunn A. R. Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA. Cell. 1979 Apr;16(4):851–861. doi: 10.1016/0092-8674(79)90100-4. [DOI] [PubMed] [Google Scholar]
  28. de Martynoff G., Pays E., Vassart G. Synthesis of a full length DNA complementary to thyroglobulin 33 S messenger RNA. Biochem Biophys Res Commun. 1980 Apr 14;93(3):645–653. doi: 10.1016/0006-291x(80)91127-4. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES