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. 1983 Apr 11;11(7):2155–2164. doi: 10.1093/nar/11.7.2155

Nuclear matrix-DNA complex resulting from EcoR1 digestion of HeLa nucleoids is enriched for DNA replicating forks

Manuel S Valenzuela 1, Gerald C Mueller 2, Santanu Dasgupta 2
PMCID: PMC325868  PMID: 6300795

Abstract

Upon treatment of HeLa nucleoids with EcoR1 restriction enzyme, a minute fraction of the DNA remains attached to the nuclear matrix. This matrix-DNA complex appears to be composed of a unique set of proteins. Electron microscopic analysis of the DNA fragments present shows an enrichment for HeLa DNA replicating forks.

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Selected References

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  1. Berezney R., Buchholtz L. A. Dynamic association of replicating DNA fragments with the nuclear matrix of regenerating liver. Exp Cell Res. 1981 Mar;132(1):1–13. doi: 10.1016/0014-4827(81)90076-8. [DOI] [PubMed] [Google Scholar]
  2. Berezney R., Coffey D. S. Identification of a nuclear protein matrix. Biochem Biophys Res Commun. 1974 Oct 23;60(4):1410–1417. doi: 10.1016/0006-291x(74)90355-6. [DOI] [PubMed] [Google Scholar]
  3. Berezney R., Coffey D. S. Nuclear protein matrix: association with newly synthesized DNA. Science. 1975 Jul 25;189(4199):291–293. doi: 10.1126/science.1145202. [DOI] [PubMed] [Google Scholar]
  4. Comings D. E., Harris D. C. Nuclear proteins. I. Electrophoretic comparison of mouse nucleoli, heterochromatin, euchromatin and contractile proteins. Exp Cell Res. 1975 Nov;96(1):161–179. doi: 10.1016/s0014-4827(75)80049-8. [DOI] [PubMed] [Google Scholar]
  5. Cook P. R., Brazell I. A., Jost E. Characterization of nuclear structures containing superhelical DNA. J Cell Sci. 1976 Nov;22(2):303–324. doi: 10.1242/jcs.22.2.303. [DOI] [PubMed] [Google Scholar]
  6. Dijkwel P. A., Mullenders L. H., Wanka F. Analysis of the attachment of replicating DNA to a nuclear matrix in mammalian interphase nuclei. Nucleic Acids Res. 1979 Jan;6(1):219–230. doi: 10.1093/nar/6.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Herlan G., Eckert W. A., Kaffenberger W., Wunderlich F. Isolation and characterization of an RNA-containing nuclear matrix from Tetrahymena macronuclei. Biochemistry. 1979 May 1;18(9):1782–1788. doi: 10.1021/bi00576a023. [DOI] [PubMed] [Google Scholar]
  8. Hodge L. D., Mancini P., Davis F. M., Heywood P. Nuclear matrix of HeLa S3 cells. Polypeptide composition during adenovirus infection and in phases of the cell cycle. J Cell Biol. 1977 Jan;72(1):194–208. doi: 10.1083/jcb.72.1.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hunt B. F., Vogelstein B. Association of newly replicated DNA with the nuclear matrix of Physarum polycephalum. Nucleic Acids Res. 1981 Jan 24;9(2):349–363. doi: 10.1093/nar/9.2.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keller J. M., Riley D. E. Nuclear ghosts: a nonmembranous structural component of mammalian cell nuclei. Science. 1976 Jul 30;193(4251):399–401. doi: 10.1126/science.935874. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Lestourgeon W. M., Forer A., Yang Y. Z., Bertram J. S., Pusch H. P. Contractile proteins. Major components of nuclear and chromosome non-histone proteins. Biochim Biophys Acta. 1975 Feb 27;379(2):529–552. [PubMed] [Google Scholar]
  13. Long B. H., Huang C. Y., Pogo A. O. Isolation and characterization of the nuclear matrix in Friend erythroleukemia cells: chromatin and hnRNA interactions with the nuclear matrix. Cell. 1979 Dec;18(4):1079–1090. doi: 10.1016/0092-8674(79)90221-6. [DOI] [PubMed] [Google Scholar]
  14. MUELLER G. C., KAJIWARA K., STUBBLEFIELD E., RUECKERT R. R. Molecular events in the reproduction of animal cells. I. The effect of puromycin on the duplication of DNA. Cancer Res. 1962 Oct;22:1084–1090. [PubMed] [Google Scholar]
  15. McCready S. J., Godwin J., Mason D. W., Brazell I. A., Cook P. R. DNA is replicated at the nuclear cage. J Cell Sci. 1980 Dec;46:365–386. doi: 10.1242/jcs.46.1.365. [DOI] [PubMed] [Google Scholar]
  16. Oho M., Riquetti P., Hudson B. G. Bovine renal glomerular basement membrane. Isolation and characterization of a glycoprotein component. J Biol Chem. 1975 Oct 10;250(19):7780–7787. [PubMed] [Google Scholar]
  17. Pardoll D. M., Vogelstein B., Coffey D. S. A fixed site of DNA replication in eucaryotic cells. Cell. 1980 Feb;19(2):527–536. doi: 10.1016/0092-8674(80)90527-9. [DOI] [PubMed] [Google Scholar]
  18. Robinson S. I., Nelkin B. D., Vogelstein B. The ovalbumin gene is associated with the nuclear matrix of chicken oviduct cells. Cell. 1982 Jan;28(1):99–106. doi: 10.1016/0092-8674(82)90379-8. [DOI] [PubMed] [Google Scholar]
  19. Sevaljević L., Petrović M., Konstantinović M., Krtolica K. Comparative studies of rat liver and sea urchin embryo nuclear matrices: partial fractionation and protein kinase activity distribution. J Cell Sci. 1982 Jun;55:189–198. doi: 10.1242/jcs.55.1.189. [DOI] [PubMed] [Google Scholar]
  20. Smith H. C., Berezney R. DNA polymerase alpha is tightly bound to the nuclear matrix of actively replicating liver. Biochem Biophys Res Commun. 1980 Dec 31;97(4):1541–1547. doi: 10.1016/s0006-291x(80)80041-6. [DOI] [PubMed] [Google Scholar]
  21. Valenzuela M. S., Aguinaga M. D., Inman R. B. Isolation of DNA fragments containing replicating growing forks from both E. coli and B. subtilis. Mol Gen Genet. 1981;181(2):241–247. doi: 10.1007/BF00268432. [DOI] [PubMed] [Google Scholar]
  22. Valenzuela M. S., Inman R. B. Restriction enzyme cleavage of DNA resulting from gently lysed Escherichia coli. Mol Gen Genet. 1978 Nov 9;166(3):245–249. doi: 10.1007/BF00267615. [DOI] [PubMed] [Google Scholar]
  23. Valenzuela M. S., Inman R. B. Visualization of a novel junction in bacteriophage lambda DNA. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3024–3028. doi: 10.1073/pnas.72.8.3024. [DOI] [PMC free article] [PubMed] [Google Scholar]

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