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. 1983 May 11;11(9):2563–2573. doi: 10.1093/nar/11.9.2563

Isolation of the messenger RNA for 8S DNA ligase in early developing axolotl egg and its cell free translation.

P Thiebaud, J Lefresne, J Signoret, J C David
PMCID: PMC325908  PMID: 6682971

Abstract

A new DNA ligase activity is expressed when the Axolotl eggs enter cleavage. The messenger RNA can be labelled by [3H] uridine thereby indicating its de novo synthesis. This new genetic expression is occurring just before cleavage and is the earliest found during Amphibian development. The newly synthesized [3H] mRNA can be translated in vitro in the rabbit reticulocyte lysate system. The resulting product is a 160 K protein specifically immunoprecipitated with the antiserum directed against 8S DNA ligase. This in vitro translated polypeptide exhibits 8S DNA ligase activity specific of activated or fertilized eggs but does not display 6S DNA ligase activity of non activated eggs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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