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. 1983 May 11;11(9):2717–2732. doi: 10.1093/nar/11.9.2717

Stability of the conservative mode of nucleosome assembly.

I M Leffak
PMCID: PMC325919  PMID: 6856473

Abstract

The conservative assembly of nucleosome histone octamer cores has been confirmed by electrophoretic analysis of density labeled histones following equilibrium buoyant density centrifugation. After normal replication, crosslinked octamers are shown not to contain a mixture of new and old core histones. Moreover, when DNA synthesis is inhibited by ara-C nucleosome cores are still assembled exclusively from nascent histone. Similarly, after release from cycloheximide inhibition newly synthesized core histone is conservatively deposited. Thus, a conservative mechanism of histone octamer assembly occurs when nascent histone is present in the normal stoichiometry to nascent DNA and when chromatin is assembled in nascent histone or nascent DNA excess.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ajiro K., Borun T. W., Cohen L. H. Phosphorylation states of different histone 1 subtypes and their relationship to chromatin functions during the HeLa S-3 cell cycle. Biochemistry. 1981 Mar 17;20(6):1445–1454. doi: 10.1021/bi00509a007. [DOI] [PubMed] [Google Scholar]
  2. Akiyama Y., Kato S. Two cell lines from lymphomas of Marek's disease. Biken J. 1974 Sep;17(3):105–116. [PubMed] [Google Scholar]
  3. Albright S. C., Wiseman J. M., Lange R. A., Garrard W. T. Subunit structures of different electrophoretic forms of nucleosomes. J Biol Chem. 1980 Apr 25;255(8):3673–3684. [PubMed] [Google Scholar]
  4. Crémisi C., Chestier A., Yaniv M. Assembly of SV40 and polyoma minichromosomes during replication. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 1):409–416. doi: 10.1101/sqb.1978.042.01.043. [DOI] [PubMed] [Google Scholar]
  5. Freedlender E. F., Taichman L., Smithies O. Nonrandom distribution of chromosomal proteins during cell replication. Biochemistry. 1977 May 3;16(9):1802–1808. doi: 10.1021/bi00628a006. [DOI] [PubMed] [Google Scholar]
  6. Gagen W. L. The significance of the "partial specific volume" obtained from sedimentation data. Biochemistry. 1966 Aug;5(8):2553–2557. doi: 10.1021/bi00872a010. [DOI] [PubMed] [Google Scholar]
  7. Goldberg R. L., Fuller G. C. Quantitation of collagen in polyacrylamide gels by fluorescent scanning of MDPF-labeled proteins: an improvement over densitometric scanning of gels stained by Coomassie Blue. Anal Biochem. 1978 Oct 1;90(1):69–80. doi: 10.1016/0003-2697(78)90009-x. [DOI] [PubMed] [Google Scholar]
  8. Gottesfeld J., Bloomer L. S. Assembly of transcriptionally active 5S RNA gene chromatin in vitro. Cell. 1982 Apr;28(4):781–791. doi: 10.1016/0092-8674(82)90057-5. [DOI] [PubMed] [Google Scholar]
  9. Hancock R. Assembly of new nucleosomal histones and new DNA into chromatin. Proc Natl Acad Sci U S A. 1978 May;75(5):2130–2134. doi: 10.1073/pnas.75.5.2130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hohmann P. Species-specific variations in H1 histone phosphopeptides. J Biol Chem. 1979 Sep 25;254(18):9022–9029. [PubMed] [Google Scholar]
  11. Jackson V., Chalkley R. A reevaluation of new histone deposition on replicating chromatin. J Biol Chem. 1981 May 25;256(10):5095–5103. [PubMed] [Google Scholar]
  12. Jackson V., Granner D., Chalkley R. Deposition of histone onto the replicating chromosome: newly synthesized histone is not found near the replication fork. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2266–2269. doi: 10.1073/pnas.73.7.2266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jackson V., Marshall S., Chalkley R. The sites of deposition of newly synthesized histone. Nucleic Acids Res. 1981 Sep 25;9(18):4563–4581. doi: 10.1093/nar/9.18.4563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  16. Leffak I. M., Grainger R., Weintraub H. Conservative assembly and segregation of nucleosomal histones. Cell. 1977 Nov;12(3):837–845. doi: 10.1016/0092-8674(77)90282-3. [DOI] [PubMed] [Google Scholar]
  17. Levinger L., Varshavsky A. High-resolution fractionation of nucleosomes: minor particles, "whiskers," and separation of mononucleosomes containing and lacking A24 semihistone. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3244–3248. doi: 10.1073/pnas.77.6.3244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mathis D., Oudet P., Chambon P. Structure of transcribing chromatin. Prog Nucleic Acid Res Mol Biol. 1980;24:1–55. doi: 10.1016/s0079-6603(08)60670-4. [DOI] [PubMed] [Google Scholar]
  19. McGhee J. D., Felsenfeld G. Nucleosome structure. Annu Rev Biochem. 1980;49:1115–1156. doi: 10.1146/annurev.bi.49.070180.005343. [DOI] [PubMed] [Google Scholar]
  20. Newrock K. M., Alfageme C. R., Nardi R. V., Cohen L. H. Histone changes during chromatin remodeling in embryogenesis. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 1):421–431. doi: 10.1101/sqb.1978.042.01.045. [DOI] [PubMed] [Google Scholar]
  21. Pospelov V., Russev G., Vassilev L., Tsanev R. Nucleosome segregation in chromatin replicated in the presence of cycloheximide. J Mol Biol. 1982 Mar 25;156(1):79–91. doi: 10.1016/0022-2836(82)90460-0. [DOI] [PubMed] [Google Scholar]
  22. Prior C. P., Cantor C. R., Johnson E. M., Allfrey V. G. Incorporation of exogenous pyrene-labeled histone into Physarum chromatin: a system for studying changes in nucleosomes assembled in vivo. Cell. 1980 Jul;20(3):597–608. doi: 10.1016/0092-8674(80)90306-2. [DOI] [PubMed] [Google Scholar]
  23. Reudelhuber T. L., Boulikas T., Garrard W. T. A nonamer of histones in chromatin. J Biol Chem. 1980 May 25;255(10):4511–4515. [PubMed] [Google Scholar]
  24. Riley D., Weintraub H. Conservative segregation of parental histones during replication in the presence of cycloheximide. Proc Natl Acad Sci U S A. 1979 Jan;76(1):328–332. doi: 10.1073/pnas.76.1.328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Roufa D. J., Marchionni M. A. Nucleosome segregation at a defined mammalian chromosomal site. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1810–1814. doi: 10.1073/pnas.79.6.1810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Roufa D. J. Replication of a mammalian genome: the role of de novo protein biosynthesis during S phase. Cell. 1978 Jan;13(1):129–138. doi: 10.1016/0092-8674(78)90144-7. [DOI] [PubMed] [Google Scholar]
  27. Russev G., Hancock R. Formation of hybrid nucleosomes cantaining new and old histones. Nucleic Acids Res. 1981 Aug 25;9(16):4129–4137. doi: 10.1093/nar/9.16.4129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Seale R. L., Simpson R. T. Effects of cycloheximide on chromatin biosynthesis. J Mol Biol. 1975 May 25;94(3):479–501. doi: 10.1016/0022-2836(75)90216-8. [DOI] [PubMed] [Google Scholar]
  29. Seale R. L. Studies on the mode of segregation of histone nu bodies during replication in HeLa cells. Cell. 1976 Nov;9(3):423–429. doi: 10.1016/0092-8674(76)90087-8. [DOI] [PubMed] [Google Scholar]
  30. Seidman M. M., Levine A. J., Weintraub H. The asymmetric segregation of parental nucleosomes during chrosome replication. Cell. 1979 Oct;18(2):439–449. doi: 10.1016/0092-8674(79)90063-1. [DOI] [PubMed] [Google Scholar]
  31. Senshu T., Fukuda M., Ohashi M. Preferential association of newly synthesized H3 and H4 histones with newly replicated DNA. J Biochem. 1978 Oct;84(4):985–988. doi: 10.1093/oxfordjournals.jbchem.a132213. [DOI] [PubMed] [Google Scholar]
  32. Stein A. DNA folding by histones: the kinetics of chromatin core particle reassembly and the interaction of nucleosomes with histones. J Mol Biol. 1979 May 15;130(2):103–134. doi: 10.1016/0022-2836(79)90421-2. [DOI] [PubMed] [Google Scholar]
  33. Trempe J., Leffak M. Assembly of semihistone A24. Nucleic Acids Res. 1982 Sep 25;10(18):5467–5481. doi: 10.1093/nar/10.18.5467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Tsanev R., Russev G. Distribution of newly synthesized histones during DNA replication. Eur J Biochem. 1974 Apr 1;43(2):257–263. doi: 10.1111/j.1432-1033.1974.tb03408.x. [DOI] [PubMed] [Google Scholar]
  35. Voordouw G., Eisenberg H. Binding of additional histones to chromatin core particles. Nature. 1978 Jun 8;273(5662):446–448. doi: 10.1038/273446a0. [DOI] [PubMed] [Google Scholar]
  36. Weintraub H. Assembly of an active chromatin structure during replication. Nucleic Acids Res. 1979 Oct 10;7(3):781–792. doi: 10.1093/nar/7.3.781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Weintraub H. Cooperative alignment of nu bodies during chromosome replication in the presence of cycloheximide. Cell. 1976 Nov;9(3):419–422. doi: 10.1016/0092-8674(76)90086-6. [DOI] [PubMed] [Google Scholar]
  38. Weintraub H., Flint S. J., Leffak I. M., Groudine M., Grainger R. M. The generation and propagation of variegated chromosome structures. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 1):401–407. doi: 10.1101/sqb.1978.042.01.042. [DOI] [PubMed] [Google Scholar]
  39. Weintraub H., Palter K., Van Lente F. Histones H2a, H2b, H3, and H4 form a tetrameric complex in solutions of high salt. Cell. 1975 Sep;6(1):85–110. doi: 10.1016/0092-8674(75)90077-x. [DOI] [PubMed] [Google Scholar]
  40. Weintraub H. The assembly of newly replicated DNA into chromatin. Cold Spring Harb Symp Quant Biol. 1974;38:247–256. doi: 10.1101/sqb.1974.038.01.028. [DOI] [PubMed] [Google Scholar]
  41. Weisbrod S., Weintraub H. Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin. Proc Natl Acad Sci U S A. 1979 Feb;76(2):630–634. doi: 10.1073/pnas.76.2.630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Woodcock C. L., Frado L. L., Wall J. S. Composition of native and reconstituted chromatin particles: direct mass determination by scanning transmission electron microscopy. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4818–4822. doi: 10.1073/pnas.77.8.4818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Worcel A., Han S., Wong M. L. Assembly of newly replicated chromatin. Cell. 1978 Nov;15(3):969–977. doi: 10.1016/0092-8674(78)90280-5. [DOI] [PubMed] [Google Scholar]

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