Table 1.
Formulationa | Length, nmb | Width, nmb | ζ-potential, mVc | Buffering Capacity, %d |
---|---|---|---|---|
PEG-CK30 | 300 ± 11 | 13 ± 0.2 | −1.4 ± 0.6 | 12 ± 5.0 |
PEG-CH12K18 | 325 ± 11 | 13 ± 0.6 | 2.0 ± 0.3** | 24 ± 2.7* |
PEI | 40 ± 3.2 | 40 ± 2.6 | 39 ± 0.6** | 23 ± 1.1* |
DNA compacted with PEG-CK30 or PEG-CH12K18 were formulated at pH 7.5 and at a final lysine to phosphate ratio (N:P) of 2:1. PEI DNA nanoparticles were formulated at pH 7.4 and at a final N:P ratio of 10:1.
DNA nanoparticle length and width as measured from TEM images, using ImageJ software package. Data represents the average of 3 independent experiments +/− SD.
Measured at pH 7.1. Data represents the average of 3 independent experiments +/− SD. * denotes statistical significance (** P < 0.001, Tukey HSD) as compared to all other groups.
P < 0.001
Buffering capacity, as measured by acid-base titration, represents the percentage of (protonable) amine groups becoming protonated from pH 7.4 to 5.1 [22]. Data represents the average of 3 independent experiments +/− SD. * denotes statistical significance (* P < 0.05, Games-Howell) as compared to PEG-CK30.
P < 0.05