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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Biomaterials. 2011 Dec 17;33(7):2361–2371. doi: 10.1016/j.biomaterials.2011.11.080

Table 1.

Physicochemical properties of DNA nanoparticles

Formulationa Length, nmb Width, nmb ζ-potential, mVc Buffering Capacity, %d
PEG-CK30 300 ± 11 13 ± 0.2 −1.4 ± 0.6 12 ± 5.0
PEG-CH12K18 325 ± 11 13 ± 0.6 2.0 ± 0.3** 24 ± 2.7*
PEI 40 ± 3.2 40 ± 2.6 39 ± 0.6** 23 ± 1.1*
a

DNA compacted with PEG-CK30 or PEG-CH12K18 were formulated at pH 7.5 and at a final lysine to phosphate ratio (N:P) of 2:1. PEI DNA nanoparticles were formulated at pH 7.4 and at a final N:P ratio of 10:1.

b

DNA nanoparticle length and width as measured from TEM images, using ImageJ software package. Data represents the average of 3 independent experiments +/− SD.

c

Measured at pH 7.1. Data represents the average of 3 independent experiments +/− SD. * denotes statistical significance (** P < 0.001, Tukey HSD) as compared to all other groups.

**

P < 0.001

d

Buffering capacity, as measured by acid-base titration, represents the percentage of (protonable) amine groups becoming protonated from pH 7.4 to 5.1 [22]. Data represents the average of 3 independent experiments +/− SD. * denotes statistical significance (* P < 0.05, Games-Howell) as compared to PEG-CK30.

*

P < 0.05