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. 1983 May 25;11(10):3027–3035. doi: 10.1093/nar/11.10.3027

Translation of lymphocyte mRNA for mouse colony stimulating factor.

R C Bleackley, H Horak, J McElhaney, C Havele, A R Shaw, A R Turner, V H Paetkau
PMCID: PMC325945  PMID: 6190128

Abstract

Poly A+ RNA has been purified from phorbol myristate acetate stimulated mouse EL4 cells. Translation, by microinjection into frog oocytes, gave biologically active interleukin 2 (IL2) and colony stimulating factor for granulocytes and macrophages (GM-CSF). These two mRNAs were separated by centrifugation through linear sucrose gradients. The sedimentation coefficients for IL2 and GM-CSF mRNAs were found to be 11.5S and 8S respectively. The clonal and cellular morphologies induced by the oocyte material corresponded to low concentrations of authentic GM-CSF.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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