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. 1983 May 25;11(10):3065–3076. doi: 10.1093/nar/11.10.3065

Cloning and expression of the Staphylococcus aureus protein A gene in Escherichia coli.

C J Duggleby, S A Jones
PMCID: PMC325948  PMID: 6304620

Abstract

A gene bank of Staphylococcus aureus strain Cowan I was established using an E. coli HB101/pBR327 host-vector system. Recombinants expressing staphylococcal protein A (SPA) were detected using an IgG-binding assay. A 3.2 Kb DNA fragment directing the synthesis of SPA in E. coli was identified. SPA produced by E. coli was characterised in minicells and by Western blotting and double diffusion experiments.

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Selected References

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