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. 1983 May 25;11(10):3077–3085. doi: 10.1093/nar/11.10.3077

Expression of human immunoglobulin E epsilon chain cDNA in E. coli.

T Kurokawa, M Seno, R Sasada, Y Ono, H Onda, K Igarashi, M Kikuchi, Y Sugino, T Honjo
PMCID: PMC325949  PMID: 6304621

Abstract

Using the cDNA of human epsilon chain, three expression plasmids that code directly the constant portion of the epsilon chain (C epsilon 1-C epsilon 4, C epsilon 2-C epsilon 4 and C epsilon 3-C epsilon 4 domains) were constructed. These epsilon chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human epsilon chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmids.

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Selected References

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