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. 1983 May 25;11(10):3393–3404. doi: 10.1093/nar/11.10.3393

Specifically alkylated DNA fragments. Synthesis and physical characterization of d[CGC(O6Me)GCG] and d[CGT(O6Me)GCG]

Sandra Kuzmich 1, Luis A Marky 1, Roger A Jones 1
PMCID: PMC325971  PMID: 6856460

Abstract

Two hexamer DNA fragments containing a carcinogenic modified base, O6-methyl guanine, have been synthesized by a solid-phase phosphotriester method, in which the unmodified guanine residues present were O6 protected with the 4-nitrophenylethyl group. These two alkylated oligonucleotides were found to have similar Tm's about 40° lower than the unmodified parent compund, d(CG)3. Moreover, the presence of the (O6Me)G appears to inhibit the B→Z transition, as determined by CD spectroscopy.

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Selected References

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