Abstract
Two hexamer DNA fragments containing a carcinogenic modified base, O6-methyl guanine, have been synthesized by a solid-phase phosphotriester method, in which the unmodified guanine residues present were O6 protected with the 4-nitrophenylethyl group. These two alkylated oligonucleotides were found to have similar Tm's about 40° lower than the unmodified parent compund, d(CG)3. Moreover, the presence of the (O6Me)G appears to inhibit the B→Z transition, as determined by CD spectroscopy.
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Selected References
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