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. 1983 Jun 25;11(12):3959–3971. doi: 10.1093/nar/11.12.3959

Detection of non-homology-containing heteroduplex molecules.

M J Lichten, M S Fox
PMCID: PMC326018  PMID: 6223275

Abstract

Heteroduplex DNA molecules which contain both the wild-type and mutant sequences of a deletion nonhomology possess a characteristic electrophoretic mobility in agarose and can be readily separated from both the wild-type and deletion-containing parental homoduplex fragments. Because of the partial single stranded character of these deletion-containing heteroduplex molecules, they are selectively bound to nitrocellulose filters, and once bound, can be selectively detected by hybridization with radioactively labeled single-stranded DNA which is homologous to the sequences absent in the deletion mutation.

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Selected References

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