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. 1983 Jun 25;11(12):4257–4272. doi: 10.1093/nar/11.12.4257

The role of queuine in the aminoacylation of mammalian aspartate transfer RNAs.

R P Singhal, V N Vakharia
PMCID: PMC326040  PMID: 6553233

Abstract

Can a queuine-specific tRNA function normally without replacement of G by Q in its structure? To answer this, kinetics of aspartate queuine-containing tRNA (Q-tRNA) is compared with its queuine-deficient counterpart (G-tRNA). The results indicate that Asp Q-tRNA is a more effective substrate than the Asp G-tRNA. The Asp Q-tRNA exhibits a higher reaction velocity (Vmax greater than 30%) and a higher reaction rate (Km less than 55%) than its counterpart. The Asp tRNAs derived from human tumor lines and grown in athymic mice contain a full complement of queuine. This tumor tRNA exhibits aminoacylation kinetics similar to a normal liver tRNA. Reasons for observing the lack of a G-to-Q modification in cancer tRNAs by others are hypothesized. Two purified Asp isoacceptors from liver are compared for the aminoacylation reaction; small differences are noted in the Vmax, but none in the Km values.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Briscoe W. T., Griffin A. C., McBride C., Bowen J. M. The distribution and properties of aspartyl transfer RNA in human and animal tumors. Cancer Res. 1975 Sep;35(9):2586–2593. [PubMed] [Google Scholar]
  2. Deutscher M. P. Aminoacyl-tRNA synthetase complex from rat liver. Methods Enzymol. 1974;29:577–583. doi: 10.1016/0076-6879(74)29051-7. [DOI] [PubMed] [Google Scholar]
  3. Dingermann T., Ogilvie A., Pistel F., Mühlhofer W., Kersten H. Reduced aminoacylation of asparagine-transfer RNA early in the developmental cycle of Dictyostelium discoideum: modification pattern and possible significance of the uncharged isoacceptor tRNAAsn3. Hoppe Seylers Z Physiol Chem. 1981 Jun;362(6):763–773. doi: 10.1515/bchm2.1981.362.1.763. [DOI] [PubMed] [Google Scholar]
  4. Frazer J. M., Yang W. K. Isoaccepting transfer ribonucleic acids in liver and brain of young and old BC3F 1 mice. Arch Biochem Biophys. 1972 Dec;153(2):610–618. doi: 10.1016/0003-9861(72)90380-3. [DOI] [PubMed] [Google Scholar]
  5. Hellman K. B., Hellman A., Cumming R. B., Novelli G. D. Comparative physiology of L-M cells propagated in vitro and in female and male mice. J Natl Cancer Inst. 1968 Sep;41(3):653–664. [PubMed] [Google Scholar]
  6. Kasai H., Kuchino Y., Nihei K., Nishimura S. Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's. Nucleic Acids Res. 1975 Oct;2(10):1931–1939. doi: 10.1093/nar/2.10.1931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Katze J. R. Alterations in SVT2 cell transfer RNAs in response to cell density and serum type. Biochim Biophys Acta. 1975 Mar 10;383(2):131–139. doi: 10.1016/0005-2787(75)90254-3. [DOI] [PubMed] [Google Scholar]
  8. Katze J. R. Alterations in SVT2 cell transfer RNAs in response to cell density and serum type. Biochim Biophys Acta. 1975 Mar 10;383(2):131–139. doi: 10.1016/0005-2787(75)90254-3. [DOI] [PubMed] [Google Scholar]
  9. Katze J. R., Basile B., McCloskey J. A. Queuine, a modified base incorporated posttranscriptionally into eukaryotic transfer RNA: wide distribution in nature. Science. 1982 Apr 2;216(4541):55–56. doi: 10.1126/science.7063869. [DOI] [PubMed] [Google Scholar]
  10. Katze J. R., Beck W. T. Administration of queuine to mice relieves modified nucleoside queuosine deficiency in Ehrlich ascites tumor tRNA. Biochem Biophys Res Commun. 1980 Sep 16;96(1):313–319. doi: 10.1016/0006-291x(80)91216-4. [DOI] [PubMed] [Google Scholar]
  11. Katze J. R., Farkas W. R. A factor in serum and amniotic fluid is a substrate for the tRNA-modifying enzyme tRNA-guanine transferase. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3271–3275. doi: 10.1073/pnas.76.7.3271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Katze J. R. Q-factor: a serum component required for the appearance of nucleoside Q in tRNA in tissue culture. Biochem Biophys Res Commun. 1978 Sep 29;84(2):527–535. doi: 10.1016/0006-291x(78)90201-2. [DOI] [PubMed] [Google Scholar]
  13. Kuchino Y., Kasai H., Nihei K., Nishimura S. Biosynthesis of the modified nucleoside Q in transfer RNA. Nucleic Acids Res. 1976 Feb;3(2):393–398. doi: 10.1093/nar/3.2.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McNamara A. L., Smith D. W. The function of the histidine tRNA isoaccepting species in hemoglobin synthesis. J Biol Chem. 1978 Sep 10;253(17):5964–5970. [PubMed] [Google Scholar]
  15. Noguchi S., Nishimura Y., Hirota Y., Nishimura S. Isolation and characterization of an Escherichia coli mutant lacking tRNA-guanine transglycosylase. Function and biosynthesis of queuosine in tRNA. J Biol Chem. 1982 Jun 10;257(11):6544–6550. [PubMed] [Google Scholar]
  16. Okada N., Shindo-Okada N., Nishimura S. Isolation of mammalian tRNAAsp and tRNATyr by lectin-Sepharose affinity column chromatography. Nucleic Acids Res. 1977 Feb;4(2):415–423. doi: 10.1093/nar/4.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Okada N., Shindo-Okada N., Sato S., Itoh Y. H., Oda K., Nishimura S. Detection of unique tRNA species in tumor tissues by Escherichia coli guanine insertion enzyme. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4247–4251. doi: 10.1073/pnas.75.9.4247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ott G., Kersten H., Nishimura S. Dictyostelium discoideum: a useful model system to evaluate the function of queuine and of the Q-family of tRNAs. FEBS Lett. 1982 Sep 20;146(2):311–314. doi: 10.1016/0014-5793(82)80941-1. [DOI] [PubMed] [Google Scholar]
  19. Reyniers J. P., Pleasants J. R., Wostmann B. S., Katze J. R., Farkas W. R. Administration of exogenous queuine is essential for the biosynthesis of the queuosine-containing transfer RNAs in the mouse. J Biol Chem. 1981 Nov 25;256(22):11591–11594. [PubMed] [Google Scholar]
  20. Roe B. A., Stankiewicz A. F., Rizi H. L., Weisz C., DiLauro M. N., Pike D., Chen C. Y., Chen E. Y. Comparison of rat liver and Walker 256 carcinosarcoma tRNAs. Nucleic Acids Res. 1979 Feb;6(2):673–688. doi: 10.1093/nar/6.2.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Saneyoshi M., Nishimura S. Selective modification of 4-thiouridylate residue in Escherichia coli transfer RNA with cyanogen bromide. Biochim Biophys Acta. 1970 Apr 15;204(2):389–399. doi: 10.1016/0005-2787(70)90158-9. [DOI] [PubMed] [Google Scholar]
  22. Shindo-Okada N., Terada M., Nishimura S. Changes in amount of hypo-modified tRNA having guanine in place of queuine during erythroid differentiation of murine erythroleukemia cells. Eur J Biochem. 1981 Apr;115(2):423–428. doi: 10.1111/j.1432-1033.1981.tb05254.x. [DOI] [PubMed] [Google Scholar]
  23. Singhal R. P. Chemical probe of structure and function of transfer ribonucleic acids. Biochemistry. 1974 Jul 2;13(14):2924–2932. doi: 10.1021/bi00711a023. [DOI] [PubMed] [Google Scholar]
  24. Singhal R. P., Fallis P. A. Structure, function, and evolution of transfer RNAs (with appendix giving complete sequences of 178 tRNAs). Prog Nucleic Acid Res Mol Biol. 1979;23:227–290. doi: 10.1016/s0079-6603(08)60135-x. [DOI] [PubMed] [Google Scholar]
  25. Singhal R. P., Griffin G. D., Novelli G. D. Separation of transfer ribonucleic acids on polystyrene anion exchangers. Biochemistry. 1976 Nov 16;15(23):5083–5087. doi: 10.1021/bi00668a021. [DOI] [PubMed] [Google Scholar]
  26. Singhal R. P., Kopper R. A., Nishimura S., Shindo-Okada N. Modification of guanine to queuine in transfer RNAs during development and aging. Biochem Biophys Res Commun. 1981 Mar 16;99(1):120–126. doi: 10.1016/0006-291x(81)91721-6. [DOI] [PubMed] [Google Scholar]
  27. Singhal R. P. Queuine: an addendum. Prog Nucleic Acid Res Mol Biol. 1983;28:75–80. doi: 10.1016/s0079-6603(08)60083-5. [DOI] [PubMed] [Google Scholar]
  28. Smith D. W., McNamara A. L. The effect of the Q base modification on the usage of tRNAHis in globin synthesis. Biochem Biophys Res Commun. 1982 Feb 26;104(4):1459–1463. doi: 10.1016/0006-291x(82)91414-0. [DOI] [PubMed] [Google Scholar]
  29. Vakharia V. N., Singhal R. P. The structure of aspartate transfer RNA from rabbit liver. Biochem Biophys Res Commun. 1982 Apr 14;105(3):1072–1081. doi: 10.1016/0006-291x(82)91079-8. [DOI] [PubMed] [Google Scholar]
  30. Wurst R. M., Vournakis J. N., Maxam A. M. Structure mapping of 5'-32P-labeled RNA with S1 nuclease. Biochemistry. 1978 Oct 17;17(21):4493–4499. doi: 10.1021/bi00614a021. [DOI] [PubMed] [Google Scholar]
  31. Yang W. K., Hellman A., Martin D. H., Hellman K. B., Novelli G. D. Isoaccepting transfer RNA's of L-M cells in culture and after tumor induction in C3H mice. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1411–1418. doi: 10.1073/pnas.64.4.1411. [DOI] [PMC free article] [PubMed] [Google Scholar]

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